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| 50g |
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Purity: ≥98%
Aspirin (Acetylsalicylic acid; ASA; Acetylin; Claradin), a widely used analgesic and a salicylate analog, is a non-selective and covalent/irreversible inhibitor of COX1 and COX2 enzymes with a broad range of biological activities such as anti-inflammatory and pain relieving effects. It has been used to relieve minor aches and pains as an analgesic agent, to reduce fever as an antipyretic agent, and to treat inflammation conditions as an anti-inflammatory drug. Aspirin also shows potent anti-proliferative activity in vitro against many cancer cell lines such as ovarian cell harboring COX-1 by acting as histone deacetylase inhibitors to upregulate the cell cycle arrest protein p21.
| ln Vitro |
In human articular chondrocytes, aspirin inhibits COX-1 and COX-2, having IC50 values of 3.57 μM and 29.3 μM, respectively [2]. By acetylating serine 530 of COX-1, aspirin inhibits platelet aggregation and prevents the production of thromboxane A in platelets [3]. By interacting with CCAAT/enhancer binding protein beta (C/EBPbeta) and its corresponding location on the COX-2 promoter/enhancer, aspirin suppresses the expression of the COX-2 protein [3]. In T cells infected with HIV, aspirin blocks the transcription from the lgκ enhancer and long terminal repeats (LTR) in an NF-κB-dependent manner [4]. Aspirin releases mitochondrial cytochrome c, triggers the ceramide pathway, activates caspases, and activates p38 MAP kinase [6].
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| ln Vivo |
In animal modeling, aspirin can be used to create models of gastrointestinal ulcers. Male adult rats with yeast fever respond significantly to aspirin (5–150 mg/kg, PO, once) [7].
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| Animal Protocol |
Animal/Disease Models: Male albino Charles River rats (200-250 g, 8 animals/group, fever was induced by 20 ml/kg of a 20 % aqueous suspension of brewer's yeast which was injected SC in the back below the nape of the neck) [7]
Doses: 5, 25, 50, 100 and 150 mg/kg Route of Administration: PO, once Experimental Results: Produced a statistically significant decrease of 0.23 ℃ at 15 min post-drug at the dose of 150 mg/kg. Antipyretic effect gradually increased in magnitude until a peak effect of 1.96 ℃ was reached at 120 min post-drug. The ED50 of aspirin was found to be 10.3 mg/kg with confidence limits of 1.8-23.0 mg/kg. The antipyretic response to aspirin is dependent on the dose of the compound administered. |
| References |
| Molecular Formula |
C9H8O4
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| Molecular Weight |
180.16
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| CAS # |
50-78-2
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| Related CAS # |
Aspirin;50-78-2
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| SMILES |
O(C(C([H])([H])[H])=O)C1=C([H])C([H])=C([H])C([H])=C1C(=O)O[H]
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| Synonyms |
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
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| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
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| Solubility (In Vivo) |
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| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.5506 mL | 27.7531 mL | 55.5062 mL | |
| 5 mM | 1.1101 mL | 5.5506 mL | 11.1012 mL | |
| 10 mM | 0.5551 mL | 2.7753 mL | 5.5506 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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