| Size | Price | Stock | Qty |
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| 250mg |
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| 500mg |
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| 5g |
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Purity: ≥98%
Asaraldehyde (also called Asaronaldehyde; 2,4,5-trimethoxy-Benzaldehyde), a naturally occuring compound extracted from carrot (Daucus carota L.) seeds, is a potent COX-2 enzyme inhibitor with potential anti-inflammatory activity. It inhibits COX-2 with an IC50 of 100 μg/mL and exhibits 17-fold selectivity for COX-2 over COX-1.
| ln Vitro |
Asarylaldehyde (2,4,5-TMBA) is a naturally occurring COX-2 inhibitor that was isolated from carrot (Daucus carota L.) seeds. At 100 μg/mL, it significantly inhibits the activity of cyclooxygenase II (COX-2) compared to three commercial nonsteroidal anti-inflammatory drugs (IC50 values: 180, 2.52, and 2.06 μg/mL, respectively). In 3T3-L1 adipocytes, 2,4,5-TMBA, a natural cyclooxygenase-2 inhibitor, inhibits adipogenesis and oromotes lipolysis. It has been shown that at a concentration of 100 μg/mL, 2,4,5-Trimethoxybenzaldehyde (2,4,5-TMBA), which is found in plant roots, seeds, and leaves, significantly inhibits the activity of cyclooxygenase-2 (COX-2). Given that COX-2 is linked to preadipocyte development, the murine 3T3-L1 cells are cultivated with 100 μg/mL of 2,4,5-TMBA both during and after the cells have fully differentiated to examine the impact of the compound on lipolysis and adipogenesis. Oil The formation of lipid droplets during differentiation was found to be inhibited by 2,4,5-TMBA, as demonstrated by Red O staining and triglyceride assay. Additionally, 2,4,5-TMBA down-regulates the protein levels of adipogenic signaling molecules and transcription factors, including MAP kinase kinase (MEK), extracellular signal-regulated kinase (ERK), CCAAT/enhancer binding protein (C/EBP)α, β, and δ, PPAR (peroxisome proliferator-activated receptor)γ, adipocyte determination and differentiation-dependent factor 1 (ADD1), and the rate-limiting enzyme for lipid synthesis, acetyl-CoA carboxylase (ACC). Treatment of completely developed adipocytes with 2,4,5-TMBA for 72 hours resulted in a considerable reduction in lipid accumulation via boosting triglyceride hydrolysis through the up-regulation of hormone-sensitive lipase (HSL) and the suppression of perilipin A (lipid droplet coating protein). Treatment of completely differentiated 3T3-L1 adipocytes with 100 μg/mL of 2,4,5-TMBA for 24, 48, or 72 hours results in a decrease in viability of 8.35, 15.54, and 27.26%, respectively. Premadiocyte cell viability is reduced by 26.46% when they are treated with 100 μg/mL of 2,4,5-TMBA for 24 hours prior to the addition of differentiation medium[1]. The most prevalent component is 2,4,5-trimethoxybenzaldehyde (TMBA), a COX-2 inhibitor that is completely absent from both its natural host, C. kanehirae wood, and its cultured broth. In fruiting bodies, 2,4,5-trimethoxybenzaldehyde (TMBA) is the main component[2].
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| References |
[1]. Wu MR, et al. 2,4,5-TMBA, a natural inhibitor of cyclooxygenase-2, suppresses adipogenesis and promotes lipolysis in 3T3-L1 adipocytes. J Agric Food Chem. 2012 Jul 25;60(29):7262-9.
[2]. Chen CC, et al. Production of a COX-2 inhibitor, 2,4,5-trimethoxybenzaldehyde, with submerged cultured Antrodia camphorata. Lett Appl Microbiol. 2007 Apr;44(4):387-92 |
| Molecular Formula |
C10H12O4
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| Molecular Weight |
196.2
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| CAS # |
4460-86-0
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| SMILES |
O=CC1=CC(OC)=C(OC)C=C1OC
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
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| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
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| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.0968 mL | 25.4842 mL | 50.9684 mL | |
| 5 mM | 1.0194 mL | 5.0968 mL | 10.1937 mL | |
| 10 mM | 0.5097 mL | 2.5484 mL | 5.0968 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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