Size | Price | Stock | Qty |
---|---|---|---|
10mg |
|
||
50mg |
|
||
100mg |
|
||
250mg |
|
||
500mg |
|
||
1g |
|
||
Other Sizes |
|
Purity: ≥98%
Apigenin (NSC-83244, LY-080400; 4',5,7-Trihydroxyflavone; Apigenol; C.I. Natural Yellow 1) is a natural flavonoid acting as a potent P450 inhibitor with various biological activity (e.g, antioxidant, anti-inflammation, anticancer). It inhibits CYP2C9 with Ki of 2 μM. Apigenin is a natural flavonoid product isolated from many plants and belongs to the flavone class that is the aglycone of several naturally occurring glycosides. Apigenin is an antioxidant plant flavanoid which contains anti-inflammatory and anticancer properties. It may induce apoptosis and may inhibit proliferation of tumor cells by arresting the cell cycle at the G2/M phase.
ln Vitro |
Ki is 2 μM reductase, cytochrome b5, and liposomes in the CYP2C9 RECO system (a purified recombinase system incorporating recombinant human CYP2C9, P450)[1]. Apigenin (4',5,7-Trihydroxyflavone) inhibits cytochrome P450 2C9 (CYP2C9). Cell proliferation is inhibited by apigenin. Apigenin's seventh growth inhibition rates (IR) at 20, 40, and 80 μM were 38%, 71%, and 99%, in that order. After being exposed to Apigenin for 24 or 48 hours, the clonogenesis of SGC-7901 cells was suppressed in a manner that was dependent on both time and dose. Following 24 and 48 hours of Apigenin treatment, the cloning efficiency of 80 μM was 9.8% and 5%, respectively, compared to 40.4% and 43.4% for the control group [2].
|
||
---|---|---|---|
ln Vivo |
Natural flavone apigenin (4',5,7-trihydroxyflavone) has a variety of biological activities, such as anti-inflammatory, neuroprotective, anti-cancer, and antioxidant capabilities. The myocardial damage caused by Adriamycin (ADR) (24 mg/kg) is lessened by apigenin (125 mg/kg and 250 mg/kg). Apigenin prevents serum aspartate aminotransferase (AST) from being released. Serum lactate dehydrogenase (LDH) release is decreased by apelin. Serum creatine kinase (CK) levels are lowered by apigenin [3].
|
||
Animal Protocol |
|
||
References |
[1]. Si D, et al. Mechanism of CYP2C9 inhibition by flavones and flavonols. Drug Metab Dispos. 2009 Mar;37(3):629-34.
[2]. Wu K, et al. Inhibitory effects of apigenin on the growth of gastric carcinoma SGC-7901 cells. World J Gastroenterol. 2005 Aug 7;11(29):4461-4. [3]. Yu W, et al. Apigenin Attenuates Adriamycin-Induced Cardiomyocyte Apoptosis via the PI3K/AKT/mTOR Pathway. Evid Based Complement Alternat Med. 2017;2017:2590676 |
Molecular Formula |
C15H10O5
|
|
---|---|---|
Molecular Weight |
270.24
|
|
CAS # |
520-36-5
|
|
Related CAS # |
Apigenin 7-glucoside;578-74-5
|
|
SMILES |
O1C(=C([H])C(C2=C(C([H])=C(C([H])=C12)O[H])O[H])=O)C1C([H])=C([H])C(=C([H])C=1[H])O[H]
|
|
Synonyms |
|
|
Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
|
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
Solubility (In Vitro) |
|
|||
---|---|---|---|---|
Solubility (In Vivo) |
|
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 3.7004 mL | 18.5021 mL | 37.0041 mL | |
5 mM | 0.7401 mL | 3.7004 mL | 7.4008 mL | |
10 mM | 0.3700 mL | 1.8502 mL | 3.7004 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.