| Size | Price | Stock | Qty |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
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Purity: ≥98%
| Targets |
CB1; CB2; PPAR; TRPV1; Microbial Metabolite; Human Endogenous Metabolite
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| ln Vitro |
Anandamide (0-250 μg/ml, 1 h) inhibits the growth of Candida albicans hyphae and stops them from adhering to epithelial cells[4].
Anandamide (0-250 μg/ml, 2 h) modifies the expression of genes related to hyphal morphogenesis and adhesion[4]. Anandamide inhibits the activity of protein kinases, which lowers tau phosphorylation[3]. |
| ln Vivo |
Anandamide (10 mg/kg, IP, once) improves glucose tolerance by significantly reducing the rise in glycemia in response to glucose ingestion when compared to control[2].
Anandamide (100 ng, ICV bilateral injection, single) partially mitigates the cognitive deficits, alterations in synaptic markers, and ventricle enlargement caused by streptozotocin (STZ)[3]. Anandamide reduces the development of inflammation by acting as an anti-inflammatory in a mouse model of ulcerative colitis[4]. Anandamide reduces lipopolysaccharide (LPS)-induced neuroinflammation in rat primary microglial cultures[1]. |
| Cell Assay |
Cell Line: HeLa cervical epithelial cells
Concentration: 0, 10, 50, 125, 250 μg/ml Incubation Time: 2 h Result: Repressed the expression of the HWP1 and ALS3 adhesins involved in Candida adhesion to epithelial cells and the HGC1, RAS1, EFG1 and ZAP1 regulators of hyphal morphogenesis and cell adherence. Increased the expression of NRG1, a transcriptional repressor of filamentous growth. |
| Animal Protocol |
Male Wistar rats (3-4 months, 350-400 g, STZ-induced AD-like sporadic dementia model)
100 ng ICV bilateral injection, single |
| References |
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| Molecular Formula |
C22H37NO2
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|---|---|
| Molecular Weight |
347.53468
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| Exact Mass |
361.26
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| Elemental Analysis |
C, 73.09; H, 9.76; N, 3.87; O, 13.28
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| CAS # |
94421-68-8
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| Appearance |
A solution in ethanol
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| SMILES |
CCCCC/C=C\C/C=C\C/C=C\C/C=C\CCCC(=O)NCCO
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| InChi Key |
LGEQQWMQCRIYKG-DOFZRALJSA-N
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| InChi Code |
InChI=1S/C22H37NO2/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-18-19-22(25)23-20-21-24/h6-7,9-10,12-13,15-16,24H,2-5,8,11,14,17-21H2,1H3,(H,23,25)/b7-6-,10-9-,13-12-,16-15-
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| Chemical Name |
(5Z,8Z,11Z,14Z)-N-(2-hydroxyethyl)icosa-5,8,11,14-tetraenamide
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| Synonyms |
Arachidonylethanolamide; Anandamide; AEA; Arachidonoyl ethanolamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: ≥ 100 mg/mL (~287.7 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (5.99 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (5.99 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (5.99 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.8774 mL | 14.3872 mL | 28.7745 mL | |
| 5 mM | 0.5755 mL | 2.8774 mL | 5.7549 mL | |
| 10 mM | 0.2877 mL | 1.4387 mL | 2.8774 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
| NCT Number | Recruitment | interventions | Conditions | Sponsor/Collaborators | Start Date | Phases |
| NCT02727023 | Completed | Other: Osteopathic Manipulative Medicine |
Anandamide Endocannabinoids |
New York Institute of Technology | December 2015 | Not Applicable |
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Products are for research use only; We do not sell to patients
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