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Description: Anacardic Acid (Hydroginkgolic acid), a natural product isolated from cashew nut shell liquid, is a potent inhibitor of p300 and p300/CBP-associated factor histone acetyltranferases, which also has antibacterial activity, antimicrobial activity, prostaglandin synthase inhibition, and tyrosinase and lipoxygenase inhibition. Anacardic acid regulates the activity and expression of several other crucial enzymes including NFκB kinase, lipoxygenase (LOX-1), xanthine oxidase, tyrosinase and ureases. Therefore, this compound exerts anti-oxidation, anti-inflammation and anti-tumor activities in vitro and in vivo.
References: J Biol Chem. 2003 May 23;278(21):19134-40; FEBS Lett. 2006 Aug 7;580(18):4353-6; PLoS One. 2014 Aug 7;9(8):e104135.
Chemical Name: 2-Hydroxy-6-pentadecyl-benzoic acid
InChi Key: ADFWQBGTDJIESE-UHFFFAOYSA-N
InChi Code: InChI=1S/C22H36O3/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-16-19-17-15-18-20(23)21(19)22(24)25/h15,17-18,23H,2-14,16H2,1H3,(H,24,25)
SMILES Code: O=C(O)C1=C(CCCCCCCCCCCCCCC)C=CC=C1O
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2
In vitro activity: Anacardic acid is found to be a common inhibitor of several clinically targeted enzymes such as NFkB kinase, histone acetyltransferase (HATs), lipoxygenase (LOX-1), xanthine oxidase, tyrosinase and ureases. Anacardic acid is also an efficient inhibitor of Tip60 in vitro.It inhibits the Tip60-dependent acetylation and activation of the ATM protein kinase in HeLa cells, and sensitizes the cells to the cytotoxic effects of radiation. Anacardic acid can effectively prevent cell damage induced by H2O2 because this can be converted to ROS, hydroxyl radicals, in the presence of metal ions
Kinase Assay: After homogenization of cardiac tissues, nucleoproteins are extracted using a Nuclear Extract Kit according to the manufacturer's instructions. HAT and HDAC activities of the nuclear protein extracts are determined using a colorimetric assay included in the HAT and HDAC assay kits.
Cell Assay: HeLa and 293T cells are grown in Dulbecco’s modified Eagle’s medium/10% fetal calf serum. SQ20B and SCC35 squamous cell carcinoma cell lines are cultured in modified eagles medium/10% fetal calf serum.For anacardic acid (EMD Biosciences, CA) exposure, cells are preincubated with anacardic acid for 40 min, irradiated, then allowed to recover for 4 h. Cells are then switched to fresh media and allowed to grow for 10 days.
J Biol Chem. 2003 May 23;278(21):19134-40; FEBS Lett. 2006 Aug 7;580(18):4353-6; PLoS One. 2014 Aug 7;9(8):e104135.
Purity ≥98%
COA
MSDS