| Size | Price | Stock | Qty |
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| 25mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| Other Sizes |
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Adenosine 5′-diphosphoribose sodium (ADP ribose sodium) is a novel nicotinamide adenine nucleotide (NAD+) metabolite, and is the most potent and primary intracellular Ca2+-permeable cation TRPM2 channel activator. Also enhances autophagy. The Ca(2+)-permeable cation channel transient receptor potential melastatin 2 (TRPM2) plays a key role in pathogen-evoked phagocyte activation, postischemic neuronal apoptosis, and glucose-evoked insulin secretion, by linking these cellular responses to oxidative stress.
| ln Vitro |
Treatment with H2O2 activates poly(ADP-ribose) (PAR) polymerase-1 (PARP-1) in mouse embryonic stem cells (MEFs) to produce sodium adenosine 5′-bisphosphate ribose (ADP-ribose), a signal that activates the TRPM2 channel and promotes Ca2+ release via extracellular Ca2+ influx and/or lysosomes. In the end, this process determines whether to trigger early or late autophagy in response to various oxidations [1]. Adenosine 5'-ribose diphosphate (ADP-ribose) binds to the distinct intracellular NUDT9 homology (NUDT9-H) domain of TRPM2, which is found at its C terminator, to activate it. Intracellular Ca2+ is a significant co-activator alongside ADPR; the TRPM2 channel only opens in the presence of both ligands [2].
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| References |
[1]. Zhang DX, et al. The potential regulatory roles of NAD(+) and its metabolism in autophagy. Metabolism. 2016 Apr;65(4):454-62.
[2]. Tóth B, et al. Pore collapse underlies irreversible inactivation of TRPM2 cation channel currents. Proc Natl Acad Sci U S A. 2012 Aug 14;109(33):13440-5. |
| Molecular Formula |
C₁₅H₂₂N₅NAO₁₄P₂
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|---|---|
| Molecular Weight |
581.30
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| CAS # |
68414-18-6
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| SMILES |
[Na+].O=C[C@H]([C@H]([C@H](COP(OP(OC[C@H]1O[C@@H](N2C=NC3=C(N=CN=C23)N)[C@H](O)[C@@H]1O)(O)=O)(O)=O)O)O)O
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| Synonyms |
Adenosine 5′diphosphoribose sodiumADP ribose sodium
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~125 mg/mL (~215.04 mM)
DMSO : ~25 mg/mL (~43.01 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (172.03 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.7203 mL | 8.6014 mL | 17.2028 mL | |
| 5 mM | 0.3441 mL | 1.7203 mL | 3.4406 mL | |
| 10 mM | 0.1720 mL | 0.8601 mL | 1.7203 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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