| Size | Price | Stock | Qty |
|---|---|---|---|
| 500mg |
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| 1g |
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| Other Sizes |
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| ln Vitro |
Adenosine 5'-monophosphate monohydrate is an A1 receptor agonist [1]. Adenosine 5'-monophosphate monohydrate (5'-AMP) at doses ranging from 25 to 400 μM caused minimal cytotoxicity in RAW264.7 cells. Adenosine 5'-monophosphate monohydrate dramatically reduced TNF-α and IL-6 mRNA expression in RAW264.7 cells. At 400 μM, adenosine 5'-monophosphate monohydrate had the strongest inhibitory effect on TNF-α and IL-6 mRNA levels. Exposing cells to adenosine 5'-monophosphate monohydrate decreases NF-κB p65 recruitment to TNF-α, IL-6, and IL-1β transcription promoters [2].
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|---|---|
| ln Vivo |
Adenosine 5'-monophosphate monohydrate (5'-AMP) treatment dramatically raised the amounts of adenosine in the liver of C57BL/6J mice. In comparison, the survival rates of mice treated with adenosine 5'-monophosphate monohydrate (n=15) were 100% (8 h) and 93.3% (24 h), whereas the survival rates of mice treated with PBS (n=15) were 60% (8 h) and 33.3% (24 h). When comparing the adenosine 5'-monophosphate monohydrate group to the vehicle group, there was a substantial decrease in the levels of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT). In the 5'-adenosine monophosphate monohydrate group, there was a decrease in the area and severity of necrosis as well as a reduction in the infiltration of inflammatory cells [2].
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| References |
[1]. Rittiner JE, et al. AMP is an adenosine A1 receptor agonist. J Biol Chem. 2012 Feb 17;287(8):5301-9.
[2]. Zhan Y, et al. Adenosine 5'-monophosphate ameliorates D-galactosamine/lipopolysaccharide-induced liver injury through an adenosine receptor-independent mechanism in mice. Cell Death Dis. 2014 Jan 9;5:e985. [3]. Ayisi NK, et al. Comparison of the antiviral effects of 5-methoxymethyldeoxyuridine-5'-monophosphate with adenine arabinoside-5'-monophosphate. Antiviral Res. 1983 Sep;3(3):161-74 |
| Molecular Formula |
C10H14N5O7P
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|---|---|
| Molecular Weight |
347.2212
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| CAS # |
18422-05-4
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| Related CAS # |
Adenosine monophosphate;61-19-8;Adenosine monophosphate-13C10,15N5 disodium;Adenosine 5'-monophosphate disodium;4578-31-8;Adenosine 5'-monophosphate-13C disodium;Adenosine 5'-monophosphate-d2 disodium;Adenosine-5'-monophosphate-15N5 disodium
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| SMILES |
P(=O)(O[H])(O[H])OC([H])([H])[C@]1([H])[C@]([H])([C@]([H])([C@]([H])(N2C([H])=NC3=C(N([H])[H])N=C([H])N=C23)O1)O[H])O[H]
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O : ~5 mg/mL (~13.69 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 5.26 mg/mL (14.40 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication (<60°C).
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.8800 mL | 14.4001 mL | 28.8002 mL | |
| 5 mM | 0.5760 mL | 2.8800 mL | 5.7600 mL | |
| 10 mM | 0.2880 mL | 1.4400 mL | 2.8800 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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