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| Other Sizes |
| Targets |
Acesulfame potassium does not have a specific pharmacological target in the traditional sense. It acts as a sweetener by binding to sweet taste receptors (T1R2/T1R3) on the tongue, triggering a signal that is perceived as sweetness. The compound is not metabolized and does not interact with metabolic pathways in the body. It is excreted unchanged in the urine. Its safety and lack of caloric content make it a popular sugar substitute for individuals with diabetes or those seeking to reduce caloric intake.
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| ln Vitro |
Acesulfame potassium exhibits in vitro activity as a sweetener in taste receptor assays. In cell-based assays using cells expressing the human sweet taste receptor (T1R2/T1R3), acesulfame potassium activates the receptor, producing a signal that correlates with sweetness perception. The compound is approximately 200 times sweeter than sucrose. It is stable under a wide range of pH and temperature conditions, making it suitable for use in various food applications. These in vitro assays confirm its sweetening activity and stability.
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| ln Vivo |
Acesulfame potassium is not used as a therapeutic agent in vivo. It is ingested as a food additive and is not absorbed or metabolized. The compound is excreted unchanged in the urine. Its lack of metabolism and caloric content make it suitable for use in sugar-free and low-calorie products. It does not affect blood glucose levels, making it safe for individuals with diabetes. In vivo studies have focused on its safety and lack of toxic effects.
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| Enzyme Assay |
In vitro assays for acesulfame potassium are typically sensory or receptor-based rather than enzyme-based. The compound’s interaction with the sweet taste receptor can be studied using cell-based assays where the receptor is expressed, and receptor activation is measured by calcium flux or other signaling readouts. Stability assays are also performed to assess the compound’s resistance to degradation under various pH, temperature, and light conditions. These assays confirm its suitability as a food additive.
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| Cell Assay |
Cellular assays for acesulfame potassium are not typically performed in the context of drug discovery, as the compound is a food additive rather than a drug. However, studies have been conducted to assess its effects on gut microbiota and potential impacts on metabolic health. These studies typically involve culturing gut bacteria in the presence of the compound and measuring changes in bacterial growth or metabolite production. Cytotoxicity assays have also been performed to ensure the compound’s safety.
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| Animal Protocol |
In vivo studies with acesulfame potassium have been conducted primarily for safety assessment. Rodent studies have evaluated the compound’s acute and chronic toxicity, carcinogenicity, and reproductive toxicity. The compound is not absorbed and is excreted unchanged, and it has been shown to be safe at levels used in food products. In humans, the compound is well-tolerated and does not cause significant adverse effects at acceptable daily intake levels. These studies have supported its approval as a food additive worldwide.
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| ADME/Pharmacokinetics |
Acesulfame potassium is not absorbed and is excreted unchanged in the urine. It does not undergo metabolism in the body. The compound is rapidly eliminated, and its half-life is short. Its pharmacokinetic profile is simple and well-characterized. The lack of metabolism and accumulation makes it a safe sweetener for regular consumption.
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| Toxicity/Toxicokinetics |
Acesulfame potassium has been extensively evaluated for safety and is approved for use as a food additive in many countries. It is not genotoxic, carcinogenic, or teratogenic at levels used in foods. The acceptable daily intake (ADI) has been established by regulatory agencies such as the FDA and EFSA. High doses may cause mild gastrointestinal discomfort, but toxicity is low. The compound is considered safe for consumption by the general population, including children and pregnant women.
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| References | |
| Additional Infomation |
Acesulfame potassium is a derivative of sulfuric acid.
Acesulfame potassium is a widely used artificial sweetener that is approximately 200 times sweeter than sugar. It is also known as acesulfame K or Ace-K. The compound is heat-stable and is often used in combination with other sweeteners to improve taste. It is used in a variety of food and beverage products, including soft drinks, baked goods, and tabletop sweeteners. The compound is not metabolized and provides no calories, making it suitable for diabetics and individuals seeking to reduce caloric intake. It has a long history of safe use as a food additive. |
| Molecular Formula |
C4H4KNO4S
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|---|---|
| Molecular Weight |
201.2422
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| Exact Mass |
200.949
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| CAS # |
55589-62-3
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| Related CAS # |
Acesulfame-d4 potassium;1623054-53-4;Acesulfame;33665-90-6
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| PubChem CID |
11074431
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| Appearance |
White to off-white solid powder
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| Density |
1.512g/cm3
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| Boiling Point |
332.7ºC at 760 mmHg
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| Melting Point |
229-232°C (dec.)
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| Flash Point |
155ºC
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| LogP |
0.476
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
11
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| Complexity |
288
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
WBZFUFAFFUEMEI-UHFFFAOYSA-M
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| InChi Code |
InChI=1S/C4H5NO4S.K/c1-3-2-4(6)5-10(7,8)9-3;/h2H,1H3,(H,5,6);/q;+1/p-1
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| Chemical Name |
potassium;6-methyl-2,2-dioxo-1-oxa-2λ6-thia-3-azanidacyclohex-5-en-4-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~248.46 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (12.42 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (12.42 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (12.42 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.9692 mL | 24.8460 mL | 49.6919 mL | |
| 5 mM | 0.9938 mL | 4.9692 mL | 9.9384 mL | |
| 10 mM | 0.4969 mL | 2.4846 mL | 4.9692 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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