| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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ACBI1 (ACBI-1) is PROTAC degrader of BAF ATPase subunits SMARCA2 and SMARCA4 (DC50 = 6 nM, 11 nM and 32 nM for SMARCA2, SMARCA4 and PBRM1 in MV-4-11 cells) with anticancer activity.It is composed of a bromodomain ligand, a linker, and the E3 ubiquitin ligase VHL. ACBI1 has anti-proliferative effects and can induceapoptosis. Italso degrades the polybromo-associated BAF (PBAF) complex member PBRM1.
| ln Vitro |
ACBI1 exhibits anti-proliferative action at 1–10,000 nM over 3–7 days [1]. SK-MEL-5 cells undergo apoptosis when exposed to ACBI1 (0.3 µM; 100 hours) [1]. The E3 ubiquitin ligase von Hippel-Lindau, a linker, and a bromodomain ligand make up ACBI1[1].
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|---|---|
| Cell Assay |
Cell proliferation assay[1]
Cell Types: MV-4-11, NCI-H1568 Cell Tested Concentrations: 0-1000 nM Incubation Duration: 3-7 days Experimental Results: demonstrated anti-proliferative activity, IC50 of MV-4 is 29, 68 nM -11, NCI-H1568 cells. Apoptosis analysis[1] Cell Types: SK-MEL-5 Cell Tested Concentrations: 0.3 µM Incubation Duration: 100 hrs (hours) Experimental Results: Induction of apoptosis in SK-MEL-5 cells. |
| References |
| Molecular Formula |
C49H58FN9O7S
|
|---|---|
| Molecular Weight |
936.1043
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| Exact Mass |
935.416
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| Elemental Analysis |
C, 62.87; H, 6.25; F, 2.03; N, 13.47; O, 11.96; S, 3.42
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| CAS # |
2375564-55-7
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| Related CAS # |
ACBI1 HCl;2375564-55-7;
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| PubChem CID |
137628619
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| Appearance |
White to yellow solid powder
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| Density |
1.4±0.1 g/cm3
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| Boiling Point |
1144.1±65.0 °C at 760 mmHg
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| Flash Point |
645.7±34.3 °C
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| Vapour Pressure |
0.0±0.3 mmHg at 25°C
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| Index of Refraction |
1.684
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| LogP |
2.45
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| Hydrogen Bond Donor Count |
5
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| Hydrogen Bond Acceptor Count |
15
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| Rotatable Bond Count |
17
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| Heavy Atom Count |
67
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| Complexity |
1650
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| Defined Atom Stereocenter Count |
3
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| SMILES |
CC1=C(SC=N1)C2=CC(=C(C=C2)CNC(=O)[C@@H]3C[C@H](CN3C(=O)[C@H](C(C)(C)C)NC(=O)C4(CC4)F)O)OCCOC5=CC=C(C=C5)CN6CCN(CC6)C7=CC(=NN=C7N)C8=CC=CC=C8O
|
| InChi Key |
IVARZBJJMMUJHI-SQKKEFIPSA-N
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| InChi Code |
InChI=1S/C49H58FN9O7S/c1-30-42(67-29-53-30)32-11-12-33(26-52-45(62)39-24-34(60)28-59(39)46(63)43(48(2,3)4)54-47(64)49(50)15-16-49)41(23-32)66-22-21-65-35-13-9-31(10-14-35)27-57-17-19-58(20-18-57)38-25-37(55-56-44(38)51)36-7-5-6-8-40(36)61/h5-14,23,25,29,34,39,43,60-61H,15-22,24,26-28H2,1-4H3,(H2,51,56)(H,52,62)(H,54,64)/t34-,39+,43-/m1/s1
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| Chemical Name |
(2S,4R)-N-[[2-[2-[4-[[4-[3-amino-6-(2-hydroxyphenyl)pyridazin-4-yl]piperazin-1-yl]methyl]phenoxy]ethoxy]-4-(4-methyl-1,3-thiazol-5-yl)phenyl]methyl]-1-[(2S)-2-[(1-fluorocyclopropanecarbonyl)amino]-3,3-dimethylbutanoyl]-4-hydroxypyrrolidine-2-carboxamide
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| Synonyms |
ACBI1; ACBI-1; ACBI 1;
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ≥ 100 mg/mL (~106.83 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (2.67 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (2.67 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.0683 mL | 5.3413 mL | 10.6826 mL | |
| 5 mM | 0.2137 mL | 1.0683 mL | 2.1365 mL | |
| 10 mM | 0.1068 mL | 0.5341 mL | 1.0683 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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