| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
ACBI1 is a novel and potent PROTAC-based degrader of BAF ATPase subunits SMARCA2 and SMARCA4, it also degrades the polybromo-associated BAF (PBAF) complex member PBRM1
| ln Vitro |
ACBI1 exhibits anti-proliferative action at 1–10,000 nM over 3–7 days[1]. SK-MEL-5 cells undergo apoptosis in response to ACBI1 (0.3 µM; 100 h)[1]. The E3 ubiquitin ligase von Hippel-Lindau, a linker, and a bromodomain ligand make up ACBI1[1].
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|---|---|
| Cell Assay |
Cell Proliferation Assay[1]
Cell Types: MV-4-11, NCI-H1568 cells Tested Concentrations: 0-1000 nM Incubation Duration: 3-7 days Experimental Results: demonstrated anti-proliferative activity with IC50 of 29, 68 nM for MV-4-11, NCI-H1568 cells, respectively. Apoptosis Analysis[1] Cell Types: SK-MEL-5 cells Tested Concentrations: 0.3 µM Incubation Duration: 100 h Experimental Results: Induced apoptosis in SK-MEL-5 cells. |
| References |
| Exact Mass |
935.42
|
|---|---|
| Elemental Analysis |
C, 62.87; H, 6.25; F, 2.03; N, 13.47; O, 11.96; S, 3.42
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| Appearance |
White to yellow solid powder
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| Synonyms |
ACBI1; ACBI-1; ACBI 1;
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (2.67 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (2.67 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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