Size | Price | Stock | Qty |
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5mg |
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10mg |
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50mg |
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100mg |
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Other Sizes |
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Targets |
L-type calcium channel
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ln Vitro |
In INS-1 beta cells, urolithin C (20–100 μM; 1 hour) administration increases glucose-induced extracellular signal-regulated kinase 1/2 (ERK1/2) activation [1]. Significant inhibition of PC12 cell growth was seen with urolithin C. Treatment with urolithin C produces abnormalities related to calcium homeostasis, accelerates the generation of reactive oxygen species (ROS) and mitochondrial membrane depolarization, and increases the release of lactate dehydrogenase (LDH) and lipid peroxidation malondialdehyde (MDA) [2]. Treatment with urolithin C causes S-phase cell cycle arrest and apoptosis [2]
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ln Vivo |
Male Wistar rats weighing 140–160 g were used to study the pharmacokinetics of urolithin C (10 mg/kg; intraperitoneally given). The terminal fraction has a half-life of 11.3 hours and a total clearance (CL/F) of 3.41 L/h/kg. The distribution's initial volume (V1/F) and steady-state volume (Vss/F) are, respectively, 0.831 L/kg and 55.6 L/kg[3].
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Cell Assay |
Western Blot Analysis[1]
Cell Types: INS-1 cells Tested Concentrations: 20 μM, 100 μM Incubation Duration: 1 hour Experimental Results: Enhanced glucose-induced extracellular signal-regulated kinases 1/2 (ERK1/2) activation. |
References |
[1]. Slimane Toubal, et al. Urolithin C Increases Glucose-Induced ERK Activation Which Contributes to Insulin Secretion. Fundam Clin Pharmacol. 2020 Feb 21.
[2]. Peipei Yin, et al. Urolithin C, a gut metabolite of ellagic acid, induces apoptosis in PC12 cells through a mitochondria-mediated pathway. RSC Advances. Issue 28, 2017. [3]. Morgane Bayle, et al. Development and Validation of a Liquid Chromatography-Electrospray Ionization-Tandem Mass Spectrometry Method for the Determination of Urolithin C in Rat Plasma and Its Application to a Pharmacokinetic Study. J Pharm Biomed Anal. 2016 Nov 30;131:33-39. |
Molecular Formula |
C13H8O5
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Molecular Weight |
244.20
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CAS # |
165393-06-6
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SMILES |
O1C(C2=C([H])C(=C(C([H])=C2C2C([H])=C([H])C(=C([H])C1=2)O[H])O[H])O[H])=O
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : 50 mg/mL (204.75 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (8.52 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (8.52 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 4.0950 mL | 20.4750 mL | 40.9500 mL | |
5 mM | 0.8190 mL | 4.0950 mL | 8.1900 mL | |
10 mM | 0.4095 mL | 2.0475 mL | 4.0950 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.