| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 5mg |
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| 10mg | |||
| Other Sizes |
| Targets |
Tubuloside B exerts anti-apoptotic effects by regulating targets related to the TNFα-induced apoptotic pathway (e.g., caspase family proteins, Bcl-2 family proteins)[1]
Tubuloside B exerts antioxidative effects by regulating targets related to the oxidative stress system (e.g., superoxide dismutase, glutathione peroxidase)[2] |
|---|---|
| ln Vitro |
TNFα-mediated apoptosis is attenuated by pretreatment with tubuloside B (1, 10, or 100 mg/L) for two hours[1].
In TNFα-induced neuronal cells, pretreatment with Tubuloside B significantly increased cell viability, reduced the apoptotic rate, downregulated the expression of pro-apoptotic proteins (e.g., caspase-3, caspase-8, Bax) and upregulated the expression of anti-apoptotic protein Bcl-2; the protein expression levels were detected by western blot, and the apoptotic rate was determined by flow cytometry (Annexin V/PI staining)[1] Tubuloside B showed dose-dependent scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals and superoxide anion radicals; it also increased the activity of antioxidant enzymes (e.g., superoxide dismutase, catalase) and reduced the level of malondialdehyde (a product of lipid peroxidation) in oxidative stress-induced cells[2] |
| Enzyme Assay |
For detecting caspase activity in TNFα-induced neuronal cells: after treatment with Tubuloside B and TNFα, cell lysates were prepared, mixed with caspase substrate (specific for caspase-3 or caspase-8) and incubated at 37°C for a specific duration; the absorbance was measured at a specific wavelength to calculate caspase activity[1]
For detecting antioxidant enzyme activity: cells treated with Tubuloside B and oxidative stress inducer were lysed, and the supernatant was collected; the supernatant was mixed with enzyme-specific substrates and reaction buffers, incubated under specific conditions, and the enzyme activity (superoxide dismutase, catalase) was calculated based on the change in absorbance or fluorescence intensity[2] |
| Cell Assay |
Neuronal cell experiment for anti-apoptotic activity: Neuronal cells were seeded in culture plates and cultured to logarithmic phase; Tubuloside B at different concentrations was added for pretreatment, followed by TNFα stimulation after a specific time; after continuous culture, cell viability was detected by MTT assay, apoptotic cells were observed by Hoechst 33258 staining, and the expression of apoptotic-related proteins was detected by western blot (including protein extraction, SDS-PAGE electrophoresis, membrane transfer, incubation with primary and secondary antibodies, and chemiluminescence detection)[1]
Cell experiment for antioxidative activity: Cells were seeded in culture plates and adapted to culture; Tubuloside B at different concentrations was added and incubated, then an oxidative stress inducer was added for treatment; after culture, the cell supernatant was collected to detect malondialdehyde content, and cell lysates were prepared to detect the activity of superoxide dismutase and catalase; additionally, DPPH radical scavenging assay was performed by mixing Tubuloside B solution with DPPH ethanol solution, incubating in the dark, and measuring absorbance at a specific wavelength[2] |
| References | |
| Additional Infomation |
It has been reported that Cistanche salsa, Cistanche tubulosa, and other organisms with relevant data all contain cistanche glycoside B. Cistanche glycoside B is a phenylethanol glycoside compound that was isolated from the traditional Chinese medicine Cistanche deserticola [2]. The anti-apoptotic effect of cistanche glycoside B on TNFα-induced neurons may be related to its regulation of the extrinsic apoptosis pathway (TNFα-mediated) and the intrinsic apoptosis pathway (Bcl-2/Bax-mediated) [1]. The antioxidant effect of cistanche glycoside B is related to its ability to scavenge free radicals and enhance the activity of the endogenous antioxidant enzyme system, thereby reducing oxidative damage to cells [2].
|
| Molecular Formula |
C31H38O16
|
|---|---|
| Molecular Weight |
666.62
|
| Exact Mass |
666.216
|
| CAS # |
112516-04-8
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| PubChem CID |
9831166
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| Appearance |
White to light yellow solid powder
|
| LogP |
0.1
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| Hydrogen Bond Donor Count |
8
|
| Hydrogen Bond Acceptor Count |
16
|
| Rotatable Bond Count |
13
|
| Heavy Atom Count |
47
|
| Complexity |
1040
|
| Defined Atom Stereocenter Count |
10
|
| SMILES |
C[C@H]1[C@@H]([C@H]([C@H]([C@@H](O1)O[C@H]2[C@@H]([C@H](O[C@H]([C@@H]2OC(=O)C)OCCC3=CC(=C(C=C3)O)O)COC(=O)/C=C/C4=CC(=C(C=C4)O)O)O)O)O)O
|
| InChi Key |
HFJIGXAMJFDVFR-OMRKUVHCSA-N
|
| InChi Code |
InChI=1S/C31H38O16/c1-14-24(38)26(40)27(41)30(44-14)47-28-25(39)22(13-43-23(37)8-5-16-3-6-18(33)20(35)11-16)46-31(29(28)45-15(2)32)42-10-9-17-4-7-19(34)21(36)12-17/h3-8,11-12,14,22,24-31,33-36,38-41H,9-10,13H2,1-2H3/b8-5+/t14-,22+,24-,25+,26+,27+,28-,29+,30-,31+/m0/s1
|
| Chemical Name |
[(2R,3R,4S,5R,6R)-5-acetyloxy-6-[2-(3,4-dihydroxyphenyl)ethoxy]-3-hydroxy-4-[(2S,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methyloxan-2-yl]oxyoxan-2-yl]methyl (E)-3-(3,4-dihydroxyphenyl)prop-2-enoate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : 10 mg/mL (15.00 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1 mg/mL (1.50 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 10.0 mg/mL clear DMSO stock solution to 400 μL of PEG300 and mix evenly; then add 50 μL of Tween-80 to the above solution and mix evenly; then add 450 μL of normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1 mg/mL (1.50 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 10.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 1 mg/mL (1.50 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.5001 mL | 7.5005 mL | 15.0011 mL | |
| 5 mM | 0.3000 mL | 1.5001 mL | 3.0002 mL | |
| 10 mM | 0.1500 mL | 0.7501 mL | 1.5001 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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