| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| 50mg | |||
| Other Sizes |
| Targets |
Bacterial dihydrofolate reductase (DHFR).
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|---|---|
| ln Vitro |
At a dose of 4 x 10-9M, tetroxoprim inhibits E. coli by 50% in vitro[1].
Tetroxoprim exhibits potent antibacterial activity by inhibiting DHFR. In vitro, at a concentration of 4 x 10-⁹ M, it causes a 50% inhibition of E. coli growth. It blocks folate metabolism in susceptible bacteria, thereby disrupting nucleotide synthesis and inhibiting bacterial growth. |
| ln Vivo |
Not available in the public domain. As a DHFR inhibitor, its in vivo efficacy would typically be assessed in animal models of bacterial infection, where it would be administered to evaluate its ability to clear the infection, often in combination with sulfonamides to achieve a synergistic effect.
|
| Enzyme Assay |
The assay measures the inhibition of bacterial DHFR enzymatic activity. The enzyme is incubated with its substrate dihydrofolate and NADPH in the presence of varying concentrations of Tetroxoprim. The decrease in NADPH oxidation, measured by absorbance at 340 nm, is used to calculate the inhibitory concentration (IC50).
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| Cell Assay |
The antibacterial activity is assessed by determining the Minimum Inhibitory Concentration (MIC) against a panel of bacterial strains, such as E. coli. Bacteria are cultured in broth media with serial dilutions of Tetroxoprim. After incubation, the MIC is the lowest concentration that visibly inhibits bacterial growth.
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| ADME/Pharmacokinetics |
Physicochemical properties: Molecular Formula = C16H22N4O4, Molecular Weight = 334.37. Purity: ≥95%.
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| References | |
| Additional Infomation |
Tetroxoprim is a dimethoxybenzene.
Tetroxoprim (CAS#: 53808-87-0) is a dihydrofolate reductase inhibitor. Its mechanism of action involves blocking the bacterial folate synthesis pathway, which is essential for the production of nucleotides and amino acids. It is often studied in combination with sulfonamides, which inhibit a different step in the same pathway, leading to a synergistic antibacterial effect. It serves as a tool for exploring antifolate mechanisms and resistance patterns. |
| Molecular Formula |
C16H22N4O4
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|---|---|
| Molecular Weight |
334.37
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| Exact Mass |
334.164
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| CAS # |
53808-87-0
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| PubChem CID |
65450
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| Appearance |
White to off-white solid powder
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| Density |
1.232g/cm3
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| Boiling Point |
564.1ºC at 760mmHg
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| Flash Point |
294.9ºC
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| Index of Refraction |
1.587
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| LogP |
2.436
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
8
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| Heavy Atom Count |
24
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| Complexity |
349
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
WSWJIZXMAUYHOE-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C16H22N4O4/c1-21-4-5-24-14-12(22-2)7-10(8-13(14)23-3)6-11-9-19-16(18)20-15(11)17/h7-9H,4-6H2,1-3H3,(H4,17,18,19,20)
|
| Chemical Name |
5-[[3,5-dimethoxy-4-(2-methoxyethoxy)phenyl]methyl]pyrimidine-2,4-diamine
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : 16.67 mg/mL (49.85 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.67 mg/mL (4.99 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 16.7 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.67 mg/mL (4.99 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 16.7 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 1.67 mg/mL (4.99 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.9907 mL | 14.9535 mL | 29.9070 mL | |
| 5 mM | 0.5981 mL | 2.9907 mL | 5.9814 mL | |
| 10 mM | 0.2991 mL | 1.4953 mL | 2.9907 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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