Size | Price | Stock | Qty |
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50mg |
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Other Sizes |
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ln Vitro |
Pyrimethanil is a fungicide classified as anilinopyrimidines, specifically a strobilurin. After being exposed to two environmentally relevant concentrations of pyrimethanil for an extended period of time, the Italian tree frog H. intermedia's tissues may undergo histological changes and exhibit a variety of toxic reactions (5 and 50 µg/L)[2]. In the medium of three-day-old cultures, pyrimethanil reduces the activity of polygalacturonase, cellulase, proteinase, and laccase. For polygalacturonase, cellulase, and proteinase, the 50% reduction in total enzyme activity (IC50) caused by pyrimethanil is roughly 0.25 μM, and for laccase, it is approximately 1.0 μM[3].
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References |
[1]. Petr Masner, et al. Possible methionine biosynthesis inhibition by pyrimidinamine fungicides. Pesticide Science
[2]. L Kanetis, et al. Characterization of genetic and biochemical mechanisms of fludioxonil and pyrimethanil resistance in field isolates of Penicillium digitatum. Phytopathology [3]. Richard J. Milling, et al. Mode of action of the anilino‐pyrimidine fungicide pyrimethanil. 2. Effects on enzyme secretion in Botrytis cinerea. Volume45, Issue1, September 1995. [4]. Salvatore D'Aquino, et al. Residue levels and effectiveness of pyrimethanil vs imazalil when using heated postharvest dip treatments for control of Penicillium decay on citrus fruit. J Agric Food Chem. 2006 Jun 28;54(13):4721-6. |
Molecular Formula |
C12H13N3
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Molecular Weight |
199.25
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CAS # |
53112-28-0
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Related CAS # |
Pyrimethanil-13C,15N2;Pyrimethanil-d5;2118244-83-8
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SMILES |
N([H])(C1C([H])=C([H])C([H])=C([H])C=1[H])C1=NC(C([H])([H])[H])=C([H])C(C([H])([H])[H])=N1
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : 130 mg/mL (652.45 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 3.25 mg/mL (16.31 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 32.5 mg/mL clear DMSO stock solution to 400 μL of PEG300 and mix evenly; then add 50 μL of Tween-80 to the above solution and mix evenly; then add 450 μL of normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 3.25 mg/mL (16.31 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 32.5 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 3.25 mg/mL (16.31 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 5.0188 mL | 25.0941 mL | 50.1882 mL | |
5 mM | 1.0038 mL | 5.0188 mL | 10.0376 mL | |
10 mM | 0.5019 mL | 2.5094 mL | 5.0188 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.