| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| Other Sizes |
| Targets |
Mitochondrial cytochrome bc1 complex (quinone outside inhibitor, QoI). Pyribencarb functions as a Qo inhibitor targeting the cytochrome b Qo site of mitochondrial complex III. It disrupts the electron transport system in fungal cells, preventing spore germination and infection.
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| ln Vitro |
Pyribencarb is an effective inhibitor of the decylubiquinol-cytochrome c reductase (UCR) activity of B. cinerea and Corynespora cassiicola, as well as the succinate cytochrome c reductase (SCR) activity of B. cinerea and Corynespora cassiicola. cinerea. Pyribencarb prevents B's UCR function. cinerea, with an estimated substrate-dependent inhibition constant of 13 nM, in a non-competitive manner [1].
Pyribencarb inhibits the production of aflatoxins in Aspergillus parasiticus with an IC50 of 0.43 µM. It inhibits both wild-type B. cinerea and strobilurin-resistant B. cinerea mitochondrial complex II. At 0.1 mg/L, the compound shows 80% inhibition against gray mould. |
| ln Vivo |
Pyribencarb demonstrates good systemic activity and translaminar movement in plants. In vivo fungicidal tests show excellent control of cucumber gray mould with 100% efficacy at 6.25 mg/L. It is effective against strains resistant to benzimidazole, dicarboxamide, and other fungicide classes.
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| Enzyme Assay |
Enzyme inhibition assays are performed using mitochondrial complex III from fungal species. Pyribencarb is incubated with the enzyme, and the inhibition of cytochrome bc1 activity is measured to confirm its QoI mechanism of action.
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| Cell Assay |
Antifungal susceptibility testing is conducted using agar dilution or broth microdilution methods against various plant pathogenic fungi, including B. cinerea and Sclerotinia sclerotiorum. Minimum inhibitory concentrations (MIC) are determined to assess the compound's spectrum of activity.
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| Animal Protocol |
In vivo fungicidal efficacy is evaluated in greenhouse and field trials on crops such as cucumber, grapes, and strawberries. Pyribencarb is applied as a foliar spray, and disease control against gray mould, scab, and sclerotinia is assessed.
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| ADME/Pharmacokinetics |
Pyribencarb has favorable physicochemical properties for agricultural use, including a log P of 3.77 and water solubility of 6.67 mg/L (20°C). These properties support its systemic activity and bioavailability in plants.
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| Toxicity/Toxicokinetics |
Pyribencarb demonstrates a favorable safety profile for crops and is considered safe for use on various agricultural crops. Comprehensive toxicological data are available from regulatory submissions for pesticide registration.
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| References |
[1]. Satoshi Kataoka, et al. Mechanism of action and selectivity of a novel fungicide, pyribencarb. J Pestic Sci, 35(2), 99-106 (2010).
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| Additional Infomation |
Pyrendicin is a carbamate, specifically the methyl ester of (2-chloro-5-{(1E)-N-[(6-methylpyridin-2-yl)methoxy]acetamyl}benzyl)carbamate. It is a fungicide with excellent activity against various plant pathogenic fungi, particularly Botrytis cinerea, which causes gray mold. It is a mitochondrial cytochrome bc1 complex inhibitor and antifungal pesticide. It is a carbamate fungicide belonging to the methyl ester class of (2-chloro-5-{N-[(6-methylpyridin-2-yl)methoxy]acetamyl}benzyl)carbamates.
Pyribencarb is registered for agricultural use in Japan and Korea for the control of gray mould, scab, sclerotinia, and other fungal diseases. It is formulated as water-dispersible granules and soluble liquids. The compound is not used in human medicine. |
| Molecular Formula |
C18H20CLN3O3
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|---|---|
| Molecular Weight |
361.83
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| Exact Mass |
361.119
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| CAS # |
799247-52-2
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| PubChem CID |
11577870
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| Appearance |
Off-white to light yellow solid powder
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| Density |
1.221g/cm3
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| Index of Refraction |
1.57
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| LogP |
4.044
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
7
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| Heavy Atom Count |
25
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| Complexity |
461
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C(/C1C=CC(Cl)=C(CNC(=O)OC)C=1)(\C)=N\OCC1C=CC=C(C)N=1
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| InChi Key |
CRFYLQMIDWBKRT-LPYMAVHISA-N
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| InChi Code |
InChI=1S/C18H20ClN3O3/c1-12-5-4-6-16(21-12)11-25-22-13(2)14-7-8-17(19)15(9-14)10-20-18(23)24-3/h4-9H,10-11H2,1-3H3,(H,20,23)/b22-13+
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| Chemical Name |
methyl N-[[2-chloro-5-[(E)-C-methyl-N-[(6-methylpyridin-2-yl)methoxy]carbonimidoyl]phenyl]methyl]carbamate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : 100 mg/mL (276.37 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (6.91 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (6.91 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (6.91 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.7637 mL | 13.8186 mL | 27.6373 mL | |
| 5 mM | 0.5527 mL | 2.7637 mL | 5.5275 mL | |
| 10 mM | 0.2764 mL | 1.3819 mL | 2.7637 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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