| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| Other Sizes |
| Targets |
Pinobanksin targets multiple cellular pathways involved in oxidative stress, inflammation, and microbial growth. As a flavonoid, it acts as an antioxidant by scavenging free radicals and chelating metal ions. The compound also exhibits antimicrobial activity against various bacterial and fungal pathogens. Its anti-inflammatory effects are mediated through the modulation of inflammatory cytokine production and signaling pathways.
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| ln Vitro |
In cell-free biochemical systems, Pinobanksin exhibits potent antioxidant activity as measured by DPPH and ABTS free radical scavenging assays. The compound's ability to chelate metal ions and inhibit lipid peroxidation can be assessed in cell-free systems. Its antimicrobial activity can be evaluated using standard disk diffusion or microdilution assays against various bacterial and fungal strains.
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| ln Vivo |
In cell-based assays, Pinobanksin exhibits antioxidant effects by reducing intracellular reactive oxygen species (ROS) levels and protecting cells from oxidative stress-induced damage. The compound's anti-inflammatory effects are evaluated by measuring the inhibition of pro-inflammatory cytokine production in immune cells. Its antimicrobial activity can be assessed in cell culture models of infection.
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| Enzyme Assay |
The cell-free assay for antioxidant activity involves measuring the compound's ability to scavenge free radicals such as DPPH (2,2-diphenyl-1-picrylhydrazyl) or ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)). The compound is incubated with the radical solution, and the decrease in absorbance is measured spectrophotometrically. The IC50 or EC50 is determined from dose-response curves. Metal chelation activity can be assessed using ferrozine-based assays.
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| Cell Assay |
Cell-based assays for Pinobanksin involve culturing relevant cell lines and treating them with the compound at concentrations ranging from 0.1 to 1000 μM. Antioxidant activity is assessed by measuring the compound's ability to protect cells from oxidative stress induced by H2O2 or other oxidants. Anti-inflammatory effects are assessed by measuring cytokine production in LPS-stimulated immune cells. Cell viability is assessed using MTT or CCK-8 assays.
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| Animal Protocol |
There is no established animal experimental protocol for Pinobanksin specifically. As a natural flavonoid with antioxidant and anti-inflammatory activities, the compound could potentially be evaluated in animal models of oxidative stress, inflammation, and infection. Typical studies involve administration of the compound to mice via oral or intraperitoneal routes. Tissue damage, oxidative stress markers, and inflammatory markers are assessed.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of Pinobanksin have not been extensively reported. As a flavonoid with a molecular weight of approximately 272.25 g/mol, the compound would be expected to have limited oral bioavailability due to poor absorption and extensive metabolism. The compound is typically metabolized by gut microbiota and undergoes phase II conjugation. Further pharmacokinetic studies would be required for therapeutic development.
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| Toxicity/Toxicokinetics |
Pinobanksin is intended for research use only and lacks established toxicity profiles for therapeutic applications. Standard laboratory safety precautions should be observed when handling this natural product. As a flavonoid with diverse biological activities, it may have effects on multiple cellular processes. The compound is generally considered to have low toxicity based on its natural occurrence in foods.
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| References | |
| Additional Infomation |
Pinobanksin is a trihydroxyflavanone with three hydroxyl substituents located at positions 3, 5, and 7. It possesses antimutagenic, antioxidant, and metabolite properties. It is a trihydroxyflavanone and also a secondary α-hydroxyketone. Pinobanksin has been reported to be found in bees, Yunnan poplar, and other organisms with relevant data.
Pinobanksin is a research-grade natural product supplied for antioxidant and anti-inflammatory research. It is not an approved pharmaceutical and has no clinical trial history. The compound is a flavonoid also known as 3,5,7-trihydroxyflavanone. It is found in various plant sources including propolis. This product is intended for research use only. |
| Molecular Formula |
C15H12O5
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|---|---|
| Molecular Weight |
272.25
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| Exact Mass |
272.068
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| CAS # |
548-82-3
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| PubChem CID |
73202
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| Appearance |
White to off-white solid powder
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| Density |
1.5±0.1 g/cm3
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| Boiling Point |
570.6±50.0 °C at 760 mmHg
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| Flash Point |
222.0±23.6 °C
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| Vapour Pressure |
0.0±1.7 mmHg at 25°C
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| Index of Refraction |
1.696
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| LogP |
3.15
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
20
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| Complexity |
365
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| Defined Atom Stereocenter Count |
2
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| SMILES |
C1=CC=C(C=C1)[C@@H]2[C@H](C(=O)C3=C(C=C(C=C3O2)O)O)O
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| InChi Key |
SUYJZKRQHBQNCA-LSDHHAIUSA-N
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| InChi Code |
InChI=1S/C15H12O5/c16-9-6-10(17)12-11(7-9)20-15(14(19)13(12)18)8-4-2-1-3-5-8/h1-7,14-17,19H/t14-,15+/m0/s1
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| Chemical Name |
(2R,3R)-3,5,7-trihydroxy-2-phenyl-2,3-dihydrochromen-4-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~367.31 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (9.18 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (9.18 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (9.18 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.6731 mL | 18.3655 mL | 36.7309 mL | |
| 5 mM | 0.7346 mL | 3.6731 mL | 7.3462 mL | |
| 10 mM | 0.3673 mL | 1.8365 mL | 3.6731 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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