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| Other Sizes |
| Targets |
Phenazine methylsulfate targets electron transfer systems in biochemical assays. As an electron acceptor, it can accept electrons from NADH and transfer them to other electron acceptors. The methosulfate group is a strong electron acceptor that enables the compound to function as an electron carrier in enzyme systems. It is widely used in biochemical assays to couple NAD(P)H oxidation to the reduction of tetrazolium salts or other acceptors.
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| ln Vitro |
Phenazine methylsulfate demonstrates in vitro activity as an electron carrier in enzyme systems. It is used in tyrosine transaminase tests and for the enzymatic determination of ethanol in blood by the colorimetric micromethod. The compound is also used as an electron transfer reactant in cell viability assays. It can be used as an electron carrier for Warburg's flavinase in the hexose monophosphate system.
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| ln Vivo |
In vivo activity of phenazine methylsulfate is not extensively documented, as the compound is primarily used as a biochemical reagent. Its electron carrier properties suggest potential for various biological applications. Further studies are needed to evaluate its in vivo efficacy and safety.
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| Enzyme Assay |
In vitro enzyme assays for phenazine methylsulfate involve using it as an electron carrier to couple NAD(P)H oxidation to the reduction of tetrazolium salts (such as MTS, INT, or NBT) or other acceptors like DCPIP. The compound's ability to accept electrons from NADH and transfer them to other acceptors is the basis for its use in various colorimetric and enzymatic assays.
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| Cell Assay |
In vitro cellular assays for phenazine methylsulfate are not typically performed, as it is primarily used as a biochemical reagent. Its role as an electron transfer reactant in cell viability assays involves its use as a component of the assay system rather than as a compound tested on cells.
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| Animal Protocol |
In vivo animal experiments for phenazine methylsulfate are not extensively documented. As a biochemical reagent, it is not intended for in vivo administration. Standard laboratory safety precautions should be followed when handling this compound.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for phenazine methylsulfate are not applicable, as it is a biochemical reagent rather than a therapeutic agent. The compound is not intended for systemic administration. It should be stored under appropriate conditions to maintain its activity.
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| Toxicity/Toxicokinetics |
Phenazine methylsulfate is not intended for human therapeutic use, and toxicological data are limited as it is a biochemical reagent. The compound should be handled with appropriate safety precautions, including the use of personal protective equipment. It is for research use only.
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| Additional Infomation |
5-Methylphenazine methyl sulfate is a nitrogen-containing heterocyclic sulfate belonging to the phenazine class of compounds. It can be used as an electron carrier for Warwick's flavinase in the hexose monophosphate system and can also be used to prepare succinate dehydrogenase.
Phenazine methylsulfate (5-Methylphenazinium methylsulfate) (CAS#: 299-11-6) has the molecular formula C14H14N2O4S and a molecular weight of 306.34. It is a heterocyclic compound functioning as an electron acceptor and carrier in enzyme systems. PMS is used in various biochemical assays, including tyrosine transaminase tests and the enzymatic determination of ethanol. |
| Molecular Formula |
C14H14N2O4S
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|---|---|
| Molecular Weight |
306.34
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| Exact Mass |
306.067
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| CAS # |
299-11-6
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| PubChem CID |
9285
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| Appearance |
Yellow to brown solid powder
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| Melting Point |
158-160 °C (dec.)(lit.)
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| LogP |
2.386
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
21
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| Complexity |
297
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| Defined Atom Stereocenter Count |
0
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| SMILES |
S(=O)(=O)([O-])OC([H])([H])[H].[N+]1(C([H])([H])[H])C2=C([H])C([H])=C([H])C([H])=C2N=C2C([H])=C([H])C([H])=C([H])C=12
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| InChi Key |
RXGJTUSBYWCRBK-UHFFFAOYSA-M
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| InChi Code |
InChI=1S/C13H11N2.CH4O4S/c1-15-12-8-4-2-6-10(12)14-11-7-3-5-9-13(11)15;1-5-6(2,3)4/h2-9H,1H3;1H3,(H,2,3,4)/q+1;/p-1
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| Chemical Name |
5-methylphenazin-5-ium;methyl sulfate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : 62.5 mg/mL (204.02 mM)
H2O : 62.5 mg/mL (204.02 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (6.79 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.08 mg/mL (6.79 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: 100 mg/mL (326.43 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.2643 mL | 16.3217 mL | 32.6435 mL | |
| 5 mM | 0.6529 mL | 3.2643 mL | 6.5287 mL | |
| 10 mM | 0.3264 mL | 1.6322 mL | 3.2643 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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