| Size | Price | Stock | Qty |
|---|---|---|---|
| 1g |
|
||
| Other Sizes |
| Targets |
The primary targets of Paclobutrazol are the enzymes involved in gibberellin biosynthesis, particularly ent-kaurene oxidase, a key enzyme in the gibberellin synthesis pathway. By inhibiting gibberellin biosynthesis, it reduces stem elongation and promotes branching and flowering in plants. The compound also has antifungal activity against various fungal pathogens. It does not have defined mammalian targets but is used primarily as a plant growth regulator.
|
|---|---|
| ln Vitro |
In mature mustard seeds, paclobutrazol (foliar spray, 5–20 μg/mL) increases total soluble sugars, carbohydrates, protein, and independent dosages. The growth of fungi, including Sirococcus clavigignenti, juglandacearum, and Ceratocystis fagacearum, is inhibited by paclobutrazol (0-19.8 μg·AI/mL) [2]. Tomato seedlings treated with paclobutrazol (5-100 μg/mL, 7–21 days) have higher quality and are resistant to early blight [3].
In vitro, Paclobutrazol demonstrates inhibition of gibberellin biosynthesis in plant systems. It also shows antifungal activity against various fungal strains. The compound's effects on plant growth have been characterized in numerous agricultural studies. In plant cell cultures, it reduces cell elongation and promotes cell division. The compound's antifungal activity is concentration-dependent and has been demonstrated in standard microbiological assays. |
| ln Vivo |
In vivo activity of Paclobutrazol has been extensively characterized in plant systems. When applied as a foliar spray (5-20 µg/mL), it increases total soluble sugars, carbohydrates, protein, and independent dosages in plants. It is transported acropetally and inhibits gibberellin biosynthesis, leading to reduced stem elongation and increased branching. The compound is used in agriculture to control plant height and improve crop yield. It does not have significant in vivo activity in mammalian systems.
|
| Enzyme Assay |
In vitro enzyme/receptor binding assays for Paclobutrazol typically involve studying its effects on gibberellin biosynthesis enzymes. Plant enzyme preparations are incubated with varying concentrations of Paclobutrazol (0.1-100 µM). The inhibition of ent-kaurene oxidase activity is measured by monitoring the conversion of substrates to products using HPLC or GC-MS. Antifungal activity is assessed using standard broth microdilution methods against fungal strains.
|
| Cell Assay |
In vitro cellular assays for Paclobutrazol involve testing its effects on plant cells and fungal pathogens. Plant cells or tissues are treated with varying concentrations of Paclobutrazol (0.1-100 µM) and monitored for growth inhibition, cell elongation, and other morphological changes. For antifungal studies, fungal cultures are treated with the compound, and the minimum inhibitory concentration (MIC) is determined. The compound demonstrates concentration-dependent effects on plant growth and fungal inhibition.
|
| Animal Protocol |
In vivo animal studies for Paclobutrazol are limited, as the compound is primarily a plant growth regulator rather than a therapeutic agent. Standard toxicology studies in rodents have been conducted for regulatory purposes. These studies typically involve oral administration at various doses (1-1000 mg/kg) to assess acute and subchronic toxicity. However, detailed protocols are not well described in the available literature. The compound is not intended for clinical use.
|
| ADME/Pharmacokinetics |
The pharmacokinetic properties of Paclobutrazol in plants include its acropetal transport and accumulation in growing tissues. The compound has a molecular weight of 293.79 and a molecular formula of C₁₅H₂₀ClN₃O. In mammals, it is metabolized and excreted, but detailed pharmacokinetic parameters are not well characterized. The compound is stable under recommended storage conditions and is used in agricultural research.
|
| Toxicity/Toxicokinetics |
The toxicity profile of Paclobutrazol has been characterized in standard toxicology studies. The compound is considered to have low to moderate toxicity in mammals. Standard toxicity studies include assessment of acute oral toxicity, dermal irritation, and repeated-dose toxicity in animal models. The compound is not intended for human use and is used exclusively in agricultural research. Further toxicological studies would be needed for therapeutic applications.
|
| Additional Infomation |
1-(4-chlorophenyl)-4,4-dimethyl-2-(1H-1,2,4-triazol-1-yl)pentane-3-ol is a triazole compound with the structure 2,2-dimethylpentane, substituted at positions 2, 3, and 5 by a hydroxyl group, a 1,2,4-triazol-1-yl group, and a p-chlorophenyl group, respectively. It belongs to the triazole class, monochlorobenzene class, and secondary alcohols.
Paclobutrazol (CAS 76738-62-0) has a molecular formula of C₁₅H₂₀ClN₃O and a molecular weight of 293.79. It is a triazole-containing plant growth retardant that inhibits gibberellin biosynthesis and has antifungal activity. The compound is transported acropetally in plants and is used in agricultural research to regulate plant growth and development. It is intended for research use only and is not approved for clinical use. |
| Molecular Formula |
C15H20CLN3O
|
|---|---|
| Molecular Weight |
293.79
|
| Exact Mass |
293.129
|
| CAS # |
76738-62-0
|
| Related CAS # |
(Rac)-Paclobutrazol-15N3;2642083-16-5;Paclobutrazol-d4
|
| PubChem CID |
616765
|
| Appearance |
White to off-white solid powder
|
| Density |
1.2±0.1 g/cm3
|
| Boiling Point |
460.9±55.0 °C at 760 mmHg
|
| Melting Point |
165-166°C
|
| Flash Point |
232.6±31.5 °C
|
| Vapour Pressure |
0.0±1.2 mmHg at 25°C
|
| Index of Refraction |
1.580
|
| LogP |
2.99
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
3
|
| Rotatable Bond Count |
5
|
| Heavy Atom Count |
20
|
| Complexity |
300
|
| Defined Atom Stereocenter Count |
0
|
| InChi Key |
RMOGWMIKYWRTKW-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C15H20ClN3O/c1-15(2,3)14(20)13(19-10-17-9-18-19)8-11-4-6-12(16)7-5-11/h4-7,9-10,13-14,20H,8H2,1-3H3
|
| Chemical Name |
1-(4-chlorophenyl)-4,4-dimethyl-2-(1,2,4-triazol-1-yl)pentan-3-ol
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : 250 mg/mL (850.95 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (7.08 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (7.08 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (7.08 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.4038 mL | 17.0190 mL | 34.0379 mL | |
| 5 mM | 0.6808 mL | 3.4038 mL | 6.8076 mL | |
| 10 mM | 0.3404 mL | 1.7019 mL | 3.4038 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
