| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| Other Sizes |
| Targets |
The target of Osthenol is monoamine oxidase A (MAO-A), with a Ki value of 0.012 μM; it shows high selectivity for MAO-A, and the Ki value for monoamine oxidase B (MAO-B) is greater than 10 μM [1]
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|---|---|
| ln Vitro |
In vitro experiments showed that Osthenol exhibited significant inhibitory activity against MAO-A. At a concentration of 0.1 μM, the inhibition rate of MAO-A was more than 90%, while the inhibition rate of MAO-B was less than 10% at the same concentration, indicating high selectivity for MAO-A [1]
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| Enzyme Assay |
For the MAO activity assay, the reaction system was prepared by mixing the enzyme solution (MAO-A or MAO-B), the substrate (tyramine or kynuramine), and different concentrations of Osthenol. The reaction mixture was incubated at 37°C for 30 minutes, and then the reaction was terminated by adding a stop solution. The amount of reaction product was detected by a spectrophotometer at a specific wavelength (e.g., 405 nm for tyramine metabolites), and the inhibition rate of Osthenol on MAO was calculated based on the absorbance value. The Ki value was determined by fitting the inhibition curve using the Lineweaver-Burk double reciprocal plot method [1]
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| References | |
| Additional Infomation |
Eustine is a hydroxycoumarin, a derivative of umbelliferone, in which the hydrogen atom at position 8 is replaced by an isopentenyl group. It is a plant metabolite and antifungal agent that is functionally related to umbelliferone. Eustine has been reported to be found in citrus (Citrus sulcata), hawthorn (Melicope semecarpifolia), and other organisms with relevant data. Eustine is an isopentenylcoumarin compound. Isopentenylcoumarins are a class of natural products with a variety of biological activities, and their inhibitory effect on monoamine oxidase (MAO) has potential application value in the treatment of neurological diseases (such as depression) [1]. This literature mainly focuses on the research of Chinese medicine components, but does not clearly describe other information related to Eustine (such as its source in Chinese medicine or potential traditional medicinal uses) [2].
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| Molecular Formula |
C14H14O3
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|---|---|
| Molecular Weight |
230.25900
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| Exact Mass |
230.094
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| CAS # |
484-14-0
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| PubChem CID |
5320318
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| Appearance |
White to yellow solid powder
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| Density |
1.203g/cm3
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| Boiling Point |
422.8ºC at 760 mmHg
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| Melting Point |
124 - 125 °C
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| Flash Point |
184.1ºC
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| Vapour Pressure |
9.53E-08mmHg at 25°C
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| Index of Refraction |
1.595
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| LogP |
3.007
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
17
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| Complexity |
353
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
RAKJVIPCCGXHHS-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C14H14O3/c1-9(2)3-6-11-12(15)7-4-10-5-8-13(16)17-14(10)11/h3-5,7-8,15H,6H2,1-2H3
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| Chemical Name |
7-hydroxy-8-(3-methylbut-2-enyl)chromen-2-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~434.29 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 2.5 mg/mL (10.86 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (10.86 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (10.86 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.3429 mL | 21.7146 mL | 43.4292 mL | |
| 5 mM | 0.8686 mL | 4.3429 mL | 8.6858 mL | |
| 10 mM | 0.4343 mL | 2.1715 mL | 4.3429 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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