| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
|
||
| 5mg |
|
||
| Other Sizes |
| Targets |
Naringenin-d4 is the deuterated form of Naringenin, which is a flavonoid with strong anti-inflammatory and antioxidant activities. Naringenin also has activity against dengue virus (DENV).
|
|---|---|
| ln Vitro |
The in vitro activity of Naringenin-d4 mirrors that of Naringenin, which displays strong anti-inflammatory and antioxidant activities. The deuterated form is used as an internal standard to accurately quantify Naringenin in cell-based experiments.
|
| ln Vivo |
In vivo, Naringenin-d4 is used as a tracer to study the pharmacokinetics and metabolism of Naringenin. Naringenin is a flavonoid with anti-inflammatory and antioxidant properties.
|
| Enzyme Assay |
The primary application of Naringenin-d4 is as an internal standard in LC-MS/MS analysis. A known amount of the labeled compound is added to biological samples (e.g., plasma or tissue). Following extraction, the sample is analyzed by LC-MS/MS.
|
| Cell Assay |
In cellular assays, Naringenin-d4 is used to quantify the intracellular concentration of Naringenin. This allows researchers to study the uptake and metabolism of this flavonoid in cell lines.
|
| Animal Protocol |
In animal studies, Naringenin-d4 is utilized as an internal standard for the quantification of Naringenin in pharmacokinetic and metabolic studies. Following administration to animal models, blood or tissue samples are collected and analyzed by LC-MS/MS.
|
| ADME/Pharmacokinetics |
Naringenin-d4 has a molecular weight of 276.28 and an isotopic purity of ≥99%.
|
| Toxicity/Toxicokinetics |
Naringenin-d4 is intended for research use only and is not for therapeutic or diagnostic use in humans. As a stable isotope, it is non-radioactive and poses minimal radiological risk. Its toxicological profile is expected to be similar to that of Naringenin, which is generally recognized as safe.
|
| References | |
| Additional Infomation |
Naringenin-d4 is a stable isotope-labeled compound used as an internal standard for the bioanalysis of Naringenin. It is a valuable tool for studying the pharmacokinetics and metabolism of this important flavonoid.
|
| Molecular Formula |
C15H12O5
|
|---|---|
| Molecular Weight |
272.252784729004
|
| Exact Mass |
276.093
|
| CAS # |
1192260-78-8
|
| Related CAS # |
Naringenin;480-41-1
|
| PubChem CID |
166642496
|
| Appearance |
White to off-white solid powder
|
| LogP |
2.4
|
| Hydrogen Bond Donor Count |
3
|
| Hydrogen Bond Acceptor Count |
5
|
| Rotatable Bond Count |
1
|
| Heavy Atom Count |
20
|
| Complexity |
363
|
| Defined Atom Stereocenter Count |
1
|
| SMILES |
O=C1C[C@@H](C2C([H])=C([H])C(O)=C([H])C=2[H])OC2=CC(O)=CC(O)=C12
|
| InChi Key |
FTVWIRXFELQLPI-MNZXJJDISA-N
|
| InChi Code |
InChI=1S/C15H12O5/c16-9-3-1-8(2-4-9)13-7-12(19)15-11(18)5-10(17)6-14(15)20-13/h1-6,13,16-18H,7H2/t13-/m0/s1/i1D,2D,3D,4D
|
| Chemical Name |
(2S)-5,7-dihydroxy-2-(2,3,5,6-tetradeuterio-4-hydroxyphenyl)-2,3-dihydrochromen-4-one
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO: ~66.7 mg/mL (~241.3 mM; with ultrasonication)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.25 mg/mL (4.52 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (12.5 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.25 mg/mL (4.52 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (12.5 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 1.25 mg/mL (4.52 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (12.5 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.6731 mL | 18.3655 mL | 36.7309 mL | |
| 5 mM | 0.7346 mL | 3.6731 mL | 7.3462 mL | |
| 10 mM | 0.3673 mL | 1.8365 mL | 3.6731 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
