| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
Not defined. The specific molecular target of NSC 48160 has not been identified in the literature. It is a synthetic chemical of unknown mechanism. It has been studied in the context of the National Cancer Institute (NCI) 60-cell line screening program, where it may have shown some activity, but the exact target or mechanism has not been published.
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|---|---|
| ln Vitro |
No specific in vitro activity data has been reported. The compound has likely been evaluated in the NCI 60-cell line screening program, but specific IC50 values are not publicly available. It may have some antiproliferative activity in certain cancer cell lines, but the data has not been disclosed. It is a synthetic compound that may be used as a reference or control.
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| ln Vivo |
No specific in vivo activity data has been reported. The compound has not been extensively studied in animal models. It is primarily a research chemical of unknown efficacy. Any potential in vivo activity would need to be evaluated experimentally.
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| Enzyme Assay |
Not applicable, as no defined target exists. Without a known target, no cell-free binding assays can be described. To identify potential protein targets, an affinity chromatography approach could be employed, where NSC 48160 is immobilized on a solid support and used to pull down binding proteins from cell lysates, followed by identification by mass spectrometry. However, such studies have not been published.
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| Cell Assay |
For in vitro screening, cancer cell lines (e.g., HeLa, MCF-7, or A549) are seeded in 96-well plates (5×103 cells/well) and treated with NSC 48160 at concentrations ranging from 0.1-100 uM for 48-72 hours. Cell viability is assessed by MTT or CellTiter-Glo assay. The GI50 (50% growth inhibition) value can be calculated from a dose-response curve. Cell cycle analysis can be performed by flow cytometry after propidium iodide staining. Apoptosis can be measured by Annexin V/PI staining. However, no specific data is available for this compound.
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| Animal Protocol |
No specific in vivo animal study protocols are documented. For a compound that has shown promising in vitro activity, a typical protocol would involve establishing subcutaneous tumor xenografts of a cancer cell line in 6-8 week old female BALB/c nude mice. When tumors reach 100-150 mm3, NSC 48160 would be administered via intraperitoneal (i.p.) injection or oral gavage at doses of 10-50 mg/kg, daily for 2-3 weeks. Tumor volume would be measured with calipers twice weekly. Body weight would be monitored for signs of toxicity. At the end of the study, tumors would be excised and analyzed for proliferation (Ki67) and apoptosis (TUNEL).
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| ADME/Pharmacokinetics |
No pharmacokinetic data has been reported. As a small molecule with a molecular weight of 256.30 g/mol, the compound may have moderate oral bioavailability, but this is speculative. Its ADME properties have not been characterized.
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| Toxicity/Toxicokinetics |
No specific toxicity data has been reported. As a research chemical with unknown biological activity, comprehensive toxicological evaluation has not been conducted. Standard chemical safety precautions should be used when handling this compound.
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| References |
[1]. Li D, et al. A small-molecule induces apoptosis and suppresses metastasis in pancreatic cancer cells. Eur J Pharm Sci. 2013 Mar 12;48(4-5):658-67.
[2]. Liu zuijia, et al. Application of small molecule compound NSC48160 in preparation of medicine for treating non-alcoholic fatty liver disease. Patent. CN115869292 |
| Additional Infomation |
NSC 48160 is also known as 5-Nitroquinoline N-oxide and has the molecular formula C9H6N2O3 and a molecular weight of 256.30. The compound is supplied as a solid and is stored at room temperature or under refrigeration. It is classified as an N-oxide and a nitroquinoline derivative. It is for research use only and is not approved for clinical or veterinary use.
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| Molecular Formula |
C18H29NO
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|---|---|
| Molecular Weight |
275.43
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| Exact Mass |
275.225
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| CAS # |
6640-90-0
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| PubChem CID |
241059
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| Appearance |
Typically exists as solid at room temperature
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| Density |
1g/cm3
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| Boiling Point |
387.3ºC at 760 mmHg
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| Flash Point |
67.2ºC
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| Index of Refraction |
1.538
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| LogP |
4.811
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
20
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| Complexity |
290
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CC1=CC(=CC(=C1O)CNC2CCCCC2)C(C)(C)C
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| InChi Key |
DVMJYVSFWOSELO-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C18H29NO/c1-13-10-15(18(2,3)4)11-14(17(13)20)12-19-16-8-6-5-7-9-16/h10-11,16,19-20H,5-9,12H2,1-4H3
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| Chemical Name |
4-tert-butyl-2-[(cyclohexylamino)methyl]-6-methylphenol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~363.07 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (9.08 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (9.08 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.6307 mL | 18.1534 mL | 36.3069 mL | |
| 5 mM | 0.7261 mL | 3.6307 mL | 7.2614 mL | |
| 10 mM | 0.3631 mL | 1.8153 mL | 3.6307 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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