| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 5mg |
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| 10mg |
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| ln Vitro |
Dendritic cells exposed to 100 μg/mL of monophosphoryl lipid A for two hours detect NF-κB activation and control TLR2 [2]. Human IL-12 production is induced by monophosphoryl lipid A (5~100 μg/mL, 24 hours; dendritic cells)[2]. When monophosphoryl lipid A is increased from 0.1 to 1 μg, the proportion of mature BMDCs likewise rises [1]. Dendritic cell surface markers are upregulated by monophosphoryl lipid A's action. Lipid A monophosphorylation improves T cell responses. Increases in monophosphoryl lipid A (CD4 T cells) stimulate calcium for T cells that have been activated [2].
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| ln Vivo |
It was established that the produced monophosphoester potentiometer A may activate APC and increase the development of Th1 and Th2 iodophor immunological responses in mice [2].
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| Cell Assay |
Western Blot Analysis[2]
Cell Types: dendritic cells Tested Concentrations: 100 μg/ml Incubation Duration: 2 hrs (hours) Experimental Results: Induces NF-κB activation and regulates TLR2. |
| References |
[1]. Cheng R, et al. Recombination Monophosphoryl Lipid A-Derived Vacosome for the Development of Preventive Cancer Vaccines. ACS Appl Mater Interfaces. 2020;12(40):44554-44562.
[2]. Ismaili J, et al. Monophosphoryl lipid A activates both human dendritic cells and T cells. J Immunol. 2002;168(2):926-932. |
| Molecular Formula |
C96H184N3O22P
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|---|---|
| Molecular Weight |
1763.5008
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| CAS # |
1246298-63-4
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| SMILES |
P(=O)([O-])(O)O[C@@H]1[C@@H](CO)O[C@H]([C@@H]([C@H]1OC(C[C@@H](CCCCCCCCCCC)OC(CCCCCCCCCCCCC)=O)=O)NC(C[C@@H](CCCCCCCCCCC)OC(CCCCCCCCCCCCC)=O)=O)OC[C@@H]1[C@H]([C@@H]([C@H]([C@@H](O)O1)NC(C[C@@H](CCCCCCCCCCC)O)=O)OC(C[C@@H](CCCCCCCCCCC)O)=O)O.[NH4+]
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~25 mg/mL (~14.18 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: 2.5 mg/mL (1.42 mM) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.5671 mL | 2.8353 mL | 5.6705 mL | |
| 5 mM | 0.1134 mL | 0.5671 mL | 1.1341 mL | |
| 10 mM | 0.0567 mL | 0.2835 mL | 0.5671 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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