| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| 50mg |
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| 100mg | |||
| Other Sizes |
| Targets |
Liver X Receptor (LXR) (No IC50, Ki, or EC50 values were described in the accessible literature content) [1]
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|---|---|
| ln Vitro |
In macrophage RAW 264.7 cells, treatment with Iristectorigenin B (isolated from Belamcanda chinensis) resulted in increased expression of ATP-binding cassette transporter A1 (ABCA1) and ATP-binding cassette transporter G1 (ABCG1). The increased expression was detected at both the protein level (via Western blot analysis) and mRNA level (via reverse transcription-polymerase chain reaction, RT-PCR), indicating that Iristectorigenin B regulates the expression of these transporters at the transcriptional level. No specific concentration-dependent data (e.g., fold change of ABCA1/ABCG1 expression at different drug concentrations) were obtainable from the accessible content. [1]
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| Cell Assay |
RAW 264.7 macrophage cells were first cultured in an appropriate medium (commonly high-glucose Dulbecco's Modified Eagle Medium, DMEM) supplemented with fetal bovine serum (FBS) and antibiotics (e.g., penicillin-streptomycin) under standard cell culture conditions (37°C, 5% CO₂). After reaching a certain confluency (typically 70%-80%), the cells were treated with different concentrations of Iristectorigenin B (specific concentrations not accessible) for a predefined incubation period (common for such experiments: 12-24 hours). Following treatment, the cells were harvested; for Western blot analysis, total cellular proteins were extracted, separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), transferred to a membrane, and probed with primary antibodies against ABCA1, ABCG1, and a loading control (e.g., β-actin), followed by incubation with secondary antibodies and detection of signal. For RT-PCR analysis, total RNA was extracted from the harvested cells, reverse-transcribed into complementary DNA (cDNA), and then amplified using specific primers for ABCA1, ABCG1, and a reference gene (e.g., glyceraldehyde-3-phosphate dehydrogenase, GAPDH) to quantify the mRNA levels of the target transporters. [1]
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| References | |
| Additional Infomation |
Irisin B has been reported in iris domestica, monopterygium insects (Monopteryx inpae), and other organisms with relevant data. Irisin B is a natural compound isolated from Belamcanda chinensis (a traditional medicinal plant). It is a liver X receptor (LXR) regulator and can increase the expression of ABCA1 and ABCG1 in RAW 264.7 macrophages, suggesting that it may play a role in regulating cholesterol efflux (a key process mediated by ABCA1 and ABCG1), which is closely related to lipid metabolism and related diseases. Due to the lack of full text, more detailed background or mechanistic information is not available. [1]
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| Molecular Formula |
C17H14O7
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|---|---|
| Molecular Weight |
330.2889
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| Exact Mass |
330.074
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| CAS # |
86849-77-6
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| PubChem CID |
5488781
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| Appearance |
Off-white to yellow solid powder
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| Density |
1.483g/cm3
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| Boiling Point |
621.2ºC at 760mmHg
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| Flash Point |
232.8ºC
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| Index of Refraction |
1.67
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| LogP |
2.594
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
24
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| Complexity |
505
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
WRZOUWHPDDOJNR-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C17H14O7/c1-22-12-4-3-8(5-10(12)18)9-7-24-13-6-11(19)17(23-2)16(21)14(13)15(9)20/h3-7,18-19,21H,1-2H3
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| Chemical Name |
5,7-dihydroxy-3-(3-hydroxy-4-methoxyphenyl)-6-methoxychromen-4-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~125 mg/mL (~378.46 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (6.30 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (6.30 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (6.30 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.0276 mL | 15.1382 mL | 30.2764 mL | |
| 5 mM | 0.6055 mL | 3.0276 mL | 6.0553 mL | |
| 10 mM | 0.3028 mL | 1.5138 mL | 3.0276 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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