| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| ln Vitro |
Fanotaprim exhibits antiproliferative and parasiticidal actions against the type I RH strain of T, with EC50 values of 13 and 7300 nM, respectively. MCF-7 and Gondii cells, in that order[1]. Fanotaprim has the capacity to stop T cells from growing. gondii strains in vitro(GT1, ME49, CTG, RUB, and VAND) with EC50s spanning 7.6~ 29.8 nM[1].
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| ln Vivo |
Fanotaprim (1–10 mg/kg; po; daily; starting on day 1 and ending on day 7) has demonstrated remarkable efficacy in managing acute infection caused by extremely pathogenic strains of T. gondii in the model used on mice[1]. The CL, Vd, and t1/2 values of fanotaprim (1 mg/kg; iv; mouse) are 10.6 mL/min/kg, 1.14 L/kg, and 3.9 hours, correspondingly[1]. F, Cmax, Tmax, and AUC0-last of 47.3%, 178 ng/mL, 0.05 hours, and 750 ng h/mL, respectively, are displayed by fanotaprim (0.83 mg/kg; po; mouse)[1].
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| Animal Protocol |
Animal/Disease Models: CD-1female mice (murine model of acute toxoplasmosis)[1]
Doses: 1-10 mg/kg Route of Administration: po; daily; beginning on day 1 through day 7 Experimental Results: Mice were monitored for survival for 30 days within termittent IVIS monitoring. At doses of 10 mg/kg Fanotaprim, qd or bid for 7 days, yielded 100% survival for 30 days. |
| References |
[1]. Hopper AT, et al. Discovery of Selective Toxoplasma gondii Dihydrofolate Reductase Inhibitors for the Treatment of Toxoplasmosis. J Med Chem. 2019;62(3):1562-1576.
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| Molecular Formula |
C19H22N8O
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|---|---|
| Molecular Weight |
378.43
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| CAS # |
2120282-75-7
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| SMILES |
O(C([H])([H])[H])C1=NC([H])=C(C([H])=N1)C1C([H])=C([H])C([H])=C(C=1[H])N1C([H])([H])C([H])([H])N(C2=C([H])N=C(N([H])[H])N=C2N([H])[H])C([H])([H])C1([H])[H]
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : 33.33 mg/mL (88.07 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (6.61 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (5.50 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (5.50 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.6425 mL | 13.2125 mL | 26.4250 mL | |
| 5 mM | 0.5285 mL | 2.6425 mL | 5.2850 mL | |
| 10 mM | 0.2642 mL | 1.3212 mL | 2.6425 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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