| Size | Price | Stock | Qty |
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| 100mg |
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| 500mg |
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| 1g |
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| Other Sizes |
| Targets |
dGTP targets DNA polymerases, serving as a substrate for DNA synthesis. It is incorporated into DNA during replication and repair. dGTP is also a target for antiviral drugs aimed at combating herpes simplex virus (HSV). Its biosynthesis from dGDP is catalyzed by nucleoside diphosphate kinase.
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| ln Vitro |
dGTP demonstrates in vitro activity as a substrate for DNA polymerases in PCR, DNA sequencing, and cloning. It is essential for DNA synthesis and repair. The compound's activity is assessed by its ability to support DNA polymerization reactions in vitro.
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| ln Vivo |
dGTP is essential for DNA synthesis in vivo and exists in all living species. It is involved in the metabolic disorder called the mitochondrial DNA depletion syndrome-3 pathway in humans. dGTP is also a target for antiviral drugs aimed at combating HSV.
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| Enzyme Assay |
dGTP is used as a substrate in DNA polymerase assays to measure polymerase activity. The assay typically measures the incorporation of radiolabeled or fluorescently labeled dGTP into DNA. Inhibition of dGTP incorporation can be used to screen for antiviral compounds targeting DNA polymerases.
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| Cell Assay |
dGTP is used in cell-free in vitro assays such as PCR, DNA sequencing, and cloning. It is added to reaction mixtures containing DNA polymerase, template DNA, primers, and other dNTPs. The compound's ability to support DNA synthesis is assessed by measuring the amplification or sequencing products.
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| Animal Protocol |
dGTP is not typically administered in animal experiments as a therapeutic agent. It is used as a research reagent in molecular biology applications. Its role in DNA synthesis makes it essential for cell proliferation and viability in all living organisms.
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| ADME/Pharmacokinetics |
dGTP is a naturally occurring nucleotide that is synthesized intracellularly. Its concentration is tightly regulated within cells. dGTP is involved in the mitochondrial DNA depletion syndrome-3 pathway in humans. The compound is used as a research reagent in molecular biology applications.
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| Toxicity/Toxicokinetics |
dGTP is a naturally occurring nucleotide and is not considered toxic at physiological concentrations. However, imbalances in dNTP pools, including dGTP, can lead to mutagenesis and genomic instability. The compound is intended for research use only. Standard laboratory safety precautions should be followed when handling this compound.
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| References | |
| Additional Infomation |
Deoxyguanosine triphosphate trisodium salt (dGTP trisodium salt; 2'-Deoxyguanosine-5'-triphosphate trisodium salt) (CAS#: 93919-41-6) has the molecular formula C10H13N5O13P3·3Na and a molecular weight of 589.13. It is a nucleoside triphosphate used in DNA synthesis, PCR, sequencing, and cloning. dGTP exists in all living species. It is for research use only.
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| Molecular Formula |
C10H13N5NA3O13P3
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|---|---|
| Molecular Weight |
573.13
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| Exact Mass |
572.941
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| CAS # |
93919-41-6
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| Related CAS # |
dGTP;2564-35-4
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| PubChem CID |
135705223
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| Appearance |
Colorless to light yellow liquid
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| LogP |
0.589
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
15
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| Rotatable Bond Count |
8
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| Heavy Atom Count |
34
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| Complexity |
876
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| Defined Atom Stereocenter Count |
3
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| SMILES |
O=C1N=C(N)NC2N([C@H]3C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O3)C=NC1=2.[Na]
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| InChi Key |
IWGGLKOTEOCWQP-BIHLCPNHSA-K
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| InChi Code |
InChI=1S/C10H16N5O13P3.3Na/c11-10-13-8-7(9(17)14-10)12-3-15(8)6-1-4(16)5(26-6)2-25-30(21,22)28-31(23,24)27-29(18,19)20;;;/h3-6,16H,1-2H2,(H,21,22)(H,23,24)(H2,18,19,20)(H3,11,13,14,17);;;/q;3*+1/p-3/t4-,5+,6+;;;/m0.../s1
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| Chemical Name |
trisodium;[[[(2R,3S,5R)-5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-oxidophosphoryl]oxy-oxidophosphoryl] hydrogen phosphate
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| Synonyms |
2'-Deoxyguanosine-5'-triphosphate trisodium salt; dGTP trisodium salt; Deoxyguanosine triphosphate trisodium salt
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~66.7 mg/mL (~116.3 mM)
DMSO : < 1 mg/mL |
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| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (174.48 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.7448 mL | 8.7240 mL | 17.4480 mL | |
| 5 mM | 0.3490 mL | 1.7448 mL | 3.4896 mL | |
| 10 mM | 0.1745 mL | 0.8724 mL | 1.7448 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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