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DMT-dU-CE Phosphoramidite is a nucleoside molecule used in DNA synthesis and DNA sequencing. The compound contains a 5'-O-dimethoxytrityl (DMT) protecting group, a 2'-deoxyuridine nucleoside, and a 3'-O-(2-cyanoethyl)-N,N-diisopropylphosphoramidite group. The DMT group protects the 5'-hydroxyl group during DNA synthesis, while the phosphoramidite group allows for the coupling of the nucleoside to the growing DNA chain. The compound is used as a building block in the solid-phase synthesis of DNA oligonucleotides.
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| ln Vitro |
DMT-dU-CE DNA synthesis and sequencing can both benefit from the usage of phosphoramidite[1].
In vitro, DMT-dU-CE Phosphoramidite is used as a building block in DNA synthesis and DNA sequencing. The compound is a nucleoside molecule that is incorporated into DNA oligonucleotides during solid-phase DNA synthesis. The DMT protecting group is removed after each coupling step to allow for the addition of the next nucleotide. The compound is essential for the controlled, stepwise synthesis of DNA sequences. |
| ln Vivo |
No detailed in vivo activity data has been published for DMT-dU-CE Phosphoramidite. The compound is a research reagent used in DNA synthesis and sequencing. It is not intended for therapeutic use. Its activity is limited to its role as a building block in DNA oligonucleotide synthesis.
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| Enzyme Assay |
The purity and identity of DMT-dU-CE Phosphoramidite can be assessed using various analytical techniques. In a typical analysis, the compound is characterized by HPLC, NMR spectroscopy, and mass spectrometry. The purity is typically ≥98%. The compound's ability to function as a phosphoramidite in DNA synthesis is confirmed by using it in standard solid-phase DNA synthesis protocols and analyzing the resulting oligonucleotides by HPLC or mass spectrometry.
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| Cell Assay |
DMT-dU-CE Phosphoramidite is used in DNA synthesis reactions. In a typical solid-phase DNA synthesis protocol, the compound is dissolved in anhydrous acetonitrile and coupled to the 5'-hydroxyl group of the growing DNA chain attached to a solid support. The coupling reaction is catalyzed by a weak acid, such as tetrazole or 5-ethylthio-1H-tetrazole. After each coupling step, the DMT protecting group is removed, and the cycle is repeated to build the desired DNA sequence.
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| Animal Protocol |
No detailed in vivo animal model data has been published for DMT-dU-CE Phosphoramidite. The compound is a research reagent used in DNA synthesis and is not intended for in vivo applications. It is typically used in laboratory settings for the synthesis of DNA oligonucleotides for research purposes.
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| ADME/Pharmacokinetics |
No detailed pharmacokinetic data has been published for DMT-dU-CE Phosphoramidite. The compound has a molecular weight of 730.79 and a molecular formula of C39H47N4O8P. It is typically stored at -20°C, protected from moisture and light. The compound is soluble in anhydrous acetonitrile, which is the solvent used in DNA synthesis.
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| Toxicity/Toxicokinetics |
No detailed toxicity data has been published for DMT-dU-CE Phosphoramidite. The compound is a research reagent used in DNA synthesis and is not intended for therapeutic use. Standard laboratory safety precautions should be followed when handling the compound. The compound is not intended for human consumption or therapeutic use.
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| References | |
| Additional Infomation |
DMT-dU-CE Phosphoramidite (CAS# 109389-30-2) is a chemically modified derivative of deoxyuridine used in DNA synthesis and DNA sequencing. It is a nucleoside molecule containing a DMT protecting group, a 2'-deoxyuridine nucleoside, and a phosphoramidite group. The compound is used as a building block in the solid-phase synthesis of DNA oligonucleotides. The molecular formula is C39H47N4O8P and molecular weight is 730.79. The compound has a purity of ≥98%.
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| Molecular Formula |
C39H47N4O8P
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| Molecular Weight |
730.79
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| Exact Mass |
730.313
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| CAS # |
109389-30-2
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| Appearance |
White to off-white solid powder
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| LogP |
7.5
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| Synonyms |
DMT-dU-CE Phosphoramidite
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~136.8 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (3.42 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (3.42 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.3684 mL | 6.8419 mL | 13.6838 mL | |
| 5 mM | 0.2737 mL | 1.3684 mL | 2.7368 mL | |
| 10 mM | 0.1368 mL | 0.6842 mL | 1.3684 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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