| Size | Price | Stock | Qty |
|---|---|---|---|
| 50mg |
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| Other Sizes |
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| ln Vitro |
HeLa growth was significantly inhibited by BAMEA-O16B/Cas9 mRNA/sgHPV18 (HeLa cells) treatment as opposed to scramble sgRNA and Cas9 mRNA delivery. BAMEA-O16B demonstrates effective RNA delivery. BAMEA-O16B exhibits efficient mRNA encapsulation. The GFP knockdown efficiency is shown by BAMEA-O16B. Cas9 mRNA delivery mediated by BAMEA-O16B has the ability to control endogenous gene expression. Compared to BAMEA-O16/RNA-treated cells, BAMEA-O16B/RNA-treated cells displayed a greater endosomal escape efficiency. RFP is efficiently expressed when BAMEA-O16B/RFP mRNA (HeLa cells) nanoparticles are used [1].
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|---|---|
| ln Vivo |
Proprotein convertase subtilisin/kexin type 9 (PCSK9) levels in mouse serum were successfully lowered by BAMEA-O16B/Cas9 mRNA/sgRNA (Iv) nanoparticles to 20% of untreated animals. Mouse serum PCSK9 is reduced by BAMEA-O16B /Cas9 mRNA/sgPCSK9 nanoparticles to 20% of DPBS injection or by BAMEA-O16B /Cas9 mRNA/scramblesgRNA nanoparticle injection [1].
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| References |
[1]. Liu J, et al. Fast and Efficient CRISPR/Cas9 Genome Editing In Vivo Enabled by Bioreducible Lipid and Messenger RNA Nanoparticles. Adv Mater. 2019;31(33):e1902575.
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| Molecular Formula |
C56H111N3O6S6
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|---|---|
| Molecular Weight |
1114.89
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| CAS # |
2490668-30-7
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| SMILES |
S(CCCCCCCCCCCC)SCCOC(CCN(CCC(=O)OCCSSCCCCCCCCCCCC)CCN(C)CCNCCC(=O)OCCSSCCCCCCCCCCCC)=O
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| Synonyms |
BAMEA-O16B
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
Ethanol : ~100 mg/mL (~89.69 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (2.24 mM) (saturation unknown) in 10% EtOH + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear EtOH stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.5 mg/mL (2.24 mM) in 10% EtOH + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear EtOH stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (2.24 mM) (saturation unknown) in 10% EtOH + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.8969 mL | 4.4847 mL | 8.9695 mL | |
| 5 mM | 0.1794 mL | 0.8969 mL | 1.7939 mL | |
| 10 mM | 0.0897 mL | 0.4485 mL | 0.8969 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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