| Size | Price | |
|---|---|---|
| 100mg | ||
| 250mg | ||
| 500mg |
| Targets |
p53
|
|---|---|
| ln Vitro |
Amifostine thiol can lessen the negative effects of both high and low LET radiation exposures, both immediately and later[1].
Amifostine thiolattenuates radiation-induced G1-phase arrest as well as DNA damage[1]. Amifostine thiol (4 mM; 30 minutes) defends RKO36 cells against chromosomal damage and degeneration brought on by ionizing radiation[1] Amifostine thiol (4 mM; 30 minutes) prevents delayed genomic instability in irradiated RKO36 cells[1]. Amifostine thiol is toxic to RKO36 cells. At milimole levels, especially after prolonged treatment[1]. RKO36 cells are not protected by amifostine thiol at 40 μM from immediate chromosomal damage and cell death, but they are protected from delayed genomic instability. Amifostine thiol activates JNK, which causes p53 to become phosphorylated at threonine 81[2]. In CHO AA8 cells, amifostine thiol alters the phosphorylation of topoisomerase IIα, changing the enzyme's activity and driving the cell cycle forward[3]. |
| References |
|
| Molecular Formula |
C5H14N2S
|
|---|---|
| Molecular Weight |
134.24
|
| CAS # |
31098-42-7
|
| Related CAS # |
Amifostine thiol dihydrochloride;14653-77-1
|
| Appearance |
Solid
|
| SMILES |
C(CN)CNCCS
|
| InChi Key |
YHPLKWQJMAYFCN-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C5H14N2S/c6-2-1-3-7-4-5-8/h7-8H,1-6H2
|
| Chemical Name |
2-(3-aminopropylamino)ethanethiol
|
| Synonyms |
Amifostine thiol; WR-1065
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO: ~250 mg/mL (~1862.3 mM)
H2O: ~100 mg/mL (~744.93 mM; Need ultrasonic) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 16.67 mg/mL (124.18 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 7.4493 mL | 37.2467 mL | 74.4934 mL | |
| 5 mM | 1.4899 mL | 7.4493 mL | 14.8987 mL | |
| 10 mM | 0.7449 mL | 3.7247 mL | 7.4493 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
|
|
|
|
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
