| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg | |||
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| Other Sizes |
| Targets |
PDE5A
Human phosphodiesterase 5 (hPDE5A) – catalytic domain. [1] |
|---|---|
| ln Vitro |
At a final concentration of 0.1 mM, Robustine exhibited 17.1% ± 0.7% inhibition of hPDE5A catalytic activity in vitro. This placed it among the less active alkaloids. Structurally, Robustine has a hydroxy group at the C-4 position (R1 = OH, R2 = H, R3 = H). The study concluded that furoquinolines with a single methoxy group at C-7 or C-8 (e.g., γ-fagarine, evolitrine) were more potent inhibitors, whereas those with a hydroxy group (like Robustine) or multiple substituents were less active or inactive. [1]
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| Enzyme Assay |
No specific enzyme assay protocol description for Robustine alone. The general hPDE5A assay used a GST-tagged catalytic domain of human PDE5A (amino acids 466-823) expressed in E. coli and purified by glutathione affinity chromatography. Activity was measured using a [³H]cGMP scintillation proximity assay (SPA). The reaction mixture (100 µL) contained 40 mM MOPS (pH 7.5), 0.5 mM EGTA, 15 mM magnesium acetate, [³H]cGMP (0.005 Ci), and 0.1 mg GST-PDE5A. The reaction was incubated for 10 min at 30°C, stopped by adding yttrium silicate beads (20 mg/mL), mixed, and incubated for 20 min at room temperature. Activity was detected on microtiter plates using a beta counter. Robustine was tested at 0.1 mM final concentration (from a 100% DMSO stock). Percent inhibition was calculated relative to control. [1]
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| References | |
| Additional Infomation |
Robustin is a quinoline alkaloid with the structure furano[2,3-b]quinoline, substituted at positions 4 and 8 with methoxy and hydroxyl groups, respectively. It is a metabolite. Robustin belongs to the quinoline alkaloid, organic heterocyclic tricyclic compound, cyclic ether, phenolic compound, and aromatic ether. It has been reported to exist in Zanthoxylum simulans, Zanthoxylum wutaiense, and other organisms with relevant data.
Robustine is a furoquinoline alkaloid isolated from Dictamnus albus. Its structure features a single hydroxy group at C-4 (R1 = OH, R2 = H, R3 = H). The study noted that the presence and position of methoxy groups are critical for PDE5A inhibitory activity; compounds with a single hydroxy group (like Robustine) or combinations of methoxy and hydroxy were inactive or less active compared to those with a single methoxy at C-8 or C-7. The IC50 for Robustine was greater than 100 µg (exact value not determined). Results were replicated using purified bovine brain phosphodiesterase (data not shown). [1] |
| Molecular Formula |
C12H9NO3
|
|---|---|
| Molecular Weight |
215.20476
|
| Exact Mass |
215.058
|
| CAS # |
2255-50-7
|
| PubChem CID |
164950
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| Appearance |
White to off-white solid
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| Density |
1.4±0.1 g/cm3
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| Boiling Point |
411.9±40.0 °C at 760 mmHg
|
| Melting Point |
148-149℃ (ethanol )
|
| Flash Point |
202.9±27.3 °C
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| Vapour Pressure |
0.0±1.0 mmHg at 25°C
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| Index of Refraction |
1.708
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| LogP |
2.73
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
4
|
| Rotatable Bond Count |
1
|
| Heavy Atom Count |
16
|
| Complexity |
261
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
COC1=C2C=COC2=NC2C(=CC=CC1=2)O
|
| InChi Key |
VGVNNMLKTSWBAR-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C12H9NO3/c1-15-11-7-3-2-4-9(14)10(7)13-12-8(11)5-6-16-12/h2-6,14H,1H3
|
| Chemical Name |
4-methoxyfuro[2,3-b]quinolin-8-ol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~464.68 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (11.62 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (11.62 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.6468 mL | 23.2342 mL | 46.4684 mL | |
| 5 mM | 0.9294 mL | 4.6468 mL | 9.2937 mL | |
| 10 mM | 0.4647 mL | 2.3234 mL | 4.6468 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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