| Size | Price | Stock | Qty |
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| 100mg |
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| 250mg |
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| 500mg |
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| Other Sizes |
| Targets |
6-FAM contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. This enables the fluorescent labeling of biomolecules through the interaction of carboxylic acid with primary amines. Fluorescein is the most common fluorescent derivatization reagent for labeling biomolecules. In addition to its relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility, fluorescein has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser.
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| ln Vitro |
In vitro, 6-FAM is widely used as a tracer and label in molecular biology for in vitro assays, cell staining, and in vivo imaging applications. It is mainly used in sequencing of nucleic acids and labeling nucleotides. FAM reagents require less stringent reaction conditions, give better conjugation yields, and the resulting conjugates have superior stability. 6-FAM is one of the most common green fluorophores used for labeling biomolecules, in particular, for labeling oligonucleotides.
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| ln Vivo |
In vivo, 6-FAM conjugates are used for imaging applications. 6-FAM conjugates are widely used in fluorescence-based nucleic acid detections such as PCR and FISH. The dye's excitation maximum closely matches the 488 nm spectral line of the argon-ion laser, making it compatible with common imaging systems.
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| Enzyme Assay |
For non-cellular assays, 6-FAM can be used to fluorescently label biomolecules through the interaction of carboxylic acid with primary amines. The labeling efficiency can be assessed by measuring fluorescence intensity at excitation/emission maxima of 492/518 nm. The compound can be used in fluorescence polarization, FRET, and other biochemical assays.
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| Cell Assay |
For in vitro cell-based assays, cells are incubated with 6-FAM-labeled probes. Fluorescence is measured using flow cytometry or fluorescence microscopy at excitation/emission of 492/518 nm. For nucleic acid detection, 6-FAM-labeled oligonucleotides are used in PCR or FISH assays. The dye can be used for cell staining and tracking.
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| Animal Protocol |
For in vivo imaging studies, 6-FAM conjugates are administered to animal models and fluorescence imaging is performed at 492/518 nm. The dye's compatibility with argon-ion laser excitation at 488 nm makes it suitable for various imaging platforms. 6-FAM conjugates are widely used in fluorescence-based nucleic acid detections.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties include solubility in DMF (1 mg/ml), DMSO (0.5 mg/ml), ethanol (5 mg/ml), and ethanol:PBS(pH 7.2) (1:1) (0.5 mg/ml). Storage is at -20°C. Purity is ≥98%. The compound is a crystalline solid. It has λmax of 226, 289 nm.
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| Toxicity/Toxicokinetics |
As a fluorescent dye, toxicity is generally low. It is not for human or veterinary use. No significant toxicity has been reported in standard research applications. The compound should be handled with standard laboratory safety precautions.
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| Additional Infomation |
6-Carboxyfluorescein is a monocarboxylic acid. It is functionally related to fluorescein (the lactone form).
6-FAM (6-Carboxyfluorescein) is a single isomer derivative of 5(6)-carboxyfluorescein. It contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation. It is mainly used in sequencing of nucleic acids and labeling nucleotides. 6-FAM is one of the most common green fluorophores used for labeling biomolecules, in particular, for labeling oligonucleotides. 6-FAM conjugates are widely used in fluorescence-based nucleic acid detections such as PCR and FISH. |
| Molecular Formula |
C21H12O7
|
|---|---|
| Molecular Weight |
376.31578
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| Exact Mass |
376.058
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| CAS # |
3301-79-9
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| Related CAS # |
6-FAM SE;92557-81-8
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| PubChem CID |
76806
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| Appearance |
Yellow to orange solid powder
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| Density |
1.7±0.1 g/cm3
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| Boiling Point |
736.4±60.0 °C at 760 mmHg
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| Melting Point |
>300 °C(lit.)
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| Flash Point |
271.0±26.4 °C
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| Vapour Pressure |
0.0±2.5 mmHg at 25°C
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| Index of Refraction |
1.816
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| LogP |
2.66
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
28
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| Complexity |
637
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
BZTDTCNHAFUJOG-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C21H12O7/c22-11-2-5-14-17(8-11)27-18-9-12(23)3-6-15(18)21(14)16-7-10(19(24)25)1-4-13(16)20(26)28-21/h1-9,22-23H,(H,24,25)
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| Chemical Name |
3',6'-dihydroxy-1-oxospiro[2-benzofuran-3,9'-xanthene]-5-carboxylic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ≥ 40 mg/mL (~106.29 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (6.64 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (6.64 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.6573 mL | 13.2866 mL | 26.5731 mL | |
| 5 mM | 0.5315 mL | 2.6573 mL | 5.3146 mL | |
| 10 mM | 0.2657 mL | 1.3287 mL | 2.6573 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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