| Size | Price | Stock | Qty |
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| 10mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| 500mg | |||
| Other Sizes |
| Targets |
6-FAM SE targets primary amines, such as those found on the N-terminus and lysine residues of proteins, as well as amino-modified nucleic acids. It does not have a specific biological target but functions as a labeling reagent for fluorescence-based detection and imaging applications.
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|---|---|
| ln Vitro |
In vitro, 6-FAM SE is characterized by its fluorescent properties, with an excitation maximum of 493 nm and an emission maximum of 517 nm. It has an extinction coefficient of 83,000 cm⁻¹ M⁻¹. It is used in nucleic acid sequencing, labeling nucleotides, and preparing fluorescent bioconjugates.
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| ln Vivo |
In vivo, 6-FAM SE is not used as a therapeutic agent. Its applications are strictly in vitro and ex vivo, including fluorescence microscopy, flow cytometry, and DNA sequencing.
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| Enzyme Assay |
The in vitro labeling protocol for 6-FAM SE involves dissolving the dye in an appropriate solvent (e.g., DMSO) and reacting it with the target molecule containing a primary amine group. The reaction is typically carried out in a bicarbonate buffer at pH 8.3-9.0, and the labeled product is purified by chromatography.
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| Cell Assay |
In vitro cell-based assays using 6-FAM SE are primarily for cell staining and tracking. Cells are incubated with the dye, which penetrates the cell membrane and covalently labels intracellular proteins. The labeled cells can then be analyzed by fluorescence microscopy or flow cytometry.
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| Animal Protocol |
In vivo animal experiments are not applicable for 6-FAM SE, as it is a fluorescent dye used for labeling and detection in vitro. It is not administered to live animals for therapeutic or experimental purposes.
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| ADME/Pharmacokinetics |
6-FAM SE has a molecular weight of 473.39 g/mol and is soluble in DMSO (~50 mg/mL). It is typically stored as a powder at -20°C, protected from light. In solution, it is stable at -80°C for 6 months and at -20°C for 1 month when protected from light.
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| Toxicity/Toxicokinetics |
6-FAM SE is considered to have low toxicity, as it is a fluorescent dye used in diagnostic and laboratory applications. However, as a research chemical, standard safety precautions should be followed. The product is not intended for therapeutic or human use.
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| Additional Infomation |
6-Carboxyfluorescein succinimide ester is an N-hydroxysuccinimide ester derived from 6-carboxyfluorescein. It is a fluorescent cell staining dye that penetrates cells and covalently couples with intracellular molecules (especially intracellular lysine residues) through its succinimide group. It functions as a fluorescent dye. Its function is related to that of fluorescein (lactone form).
6-FAM SE is a widely used research reagent with no clinical approval. It is a key tool in molecular biology for labeling nucleotides and nucleic acids, and for preparing fluorescent antibody and avidin derivatives. It is also used in automated DNA sequencing applications. |
| Molecular Formula |
C25H15NO9
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|---|---|
| Molecular Weight |
473.3879
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| Exact Mass |
473.074
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| CAS # |
92557-81-8
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| Related CAS # |
6-FAM;3301-79-9
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| PubChem CID |
2762614
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| Appearance |
Yellow to orange solid powder
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| Density |
1.7±0.1 g/cm3
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| Boiling Point |
788.1±70.0 °C at 760 mmHg
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| Flash Point |
430.4±35.7 °C
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| Vapour Pressure |
0.0±2.8 mmHg at 25°C
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| Index of Refraction |
1.790
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| LogP |
0.71
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
9
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
35
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| Complexity |
894
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
VDABVNMGKGUPEY-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C25H15NO9/c27-13-2-5-16-19(10-13)33-20-11-14(28)3-6-17(20)25(16)18-9-12(1-4-15(18)24(32)34-25)23(31)35-26-21(29)7-8-22(26)30/h1-6,9-11,27-28H,7-8H2
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| Chemical Name |
(2,5-dioxopyrrolidin-1-yl) 3',6'-dihydroxy-1-oxospiro[2-benzofuran-3,9'-xanthene]-5-carboxylate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~105.62 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (5.28 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (5.28 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.1124 mL | 10.5621 mL | 21.1242 mL | |
| 5 mM | 0.4225 mL | 2.1124 mL | 4.2248 mL | |
| 10 mM | 0.2112 mL | 1.0562 mL | 2.1124 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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