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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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5,7,4'-Trimethoxyflavone is a naturally occuring flavnoid extracted from Kaempferia parviflora (KP) that is a famous medicinal plant from Thailand. Increases in the sub-G1 phase, DNA fragmentation, annexin-V/PI staining, the Bax/Bcl-xL ratio, proteolytic activation of caspase-3, and the degradation of poly (ADP-ribose) polymerase (PARP) protein are all signs that 5,7,4'-trimethoxyflavone causes apoptosis. In a concentration-dependent manner, 5,7,4'-trimethoxyflavone significantly inhibits the growth of SNU-16 human gastric cancer cells.
| Targets |
Caspase-3; PARP
|
|---|---|
| ln Vitro |
In vitro, 5,7,4'-Trimethoxyflavone (12.5, 25, 50, 100, 200 μM, 24, 48 h) suppresses SUN-16 cell division [1]. 11, 25, 5,7,4'-Trimethoxyflavone endoplasmic reticulum and associated regulatory proteins to 5,7,4'-trimethoxyflavone (6.25, 12.5 μM, 24 h) in SUN-16 cells yields cytostatic effects[1]. The activity of ROS and pro-inflammatory regulatory factors caused by TNF-α in HDFs cells can be inhibited by 5,7,4'-Trimethoxyflavone (6.25, 12.5 μM, 24 h).
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| Cell Assay |
Cell Viability Assay[1]
Cell Types: AGS, SNU-1, SNU-16 Tested Concentrations: 12.5 ,25, 50, 100, 200 μM Incubation Duration: 24 and 48 hrs (hours) Experimental Results: demonstrated the highest results in SNU-16 cells toxicity. Apoptosis analysis [1] Cell Types: SNU-16 Tested Concentrations: 12.5, 25, 37.5, 50 μM Incubation Duration: 24 h Experimental Results: The percentage of annexin V-positive SNU-16 cells increased from 7.2% to 58.0%, and GRP78, IRE1a , ATF-4 and CHOP. Western Blot Analysis[2] Cell Types: HDF Tested Concentrations: 6.25, 12.5 μM Incubation Duration: 24 hrs (hours) Experimental Results: Inhibits matrix metalloproteinase-1 (MMP-1) expression and stimulates collagen, type I and alpha 1 (COLIA1) expression. |
| References |
[1]. Kim H, et al. Induction of ER Stress-Mediated Apoptosis by the Major Component 5,7,4'-TrimethoxyflavoneIsolated from Kaempferia parviflora Tea Infusion. Nutr Cancer. 2018 Aug-Sep;70(6):984-996.
[2]. Phung HM,et al. Protective Effect of Polymethoxyflavones Isolated from Kaempferia parviflora against TNF-α-Induced Human Dermal Fibroblast Damage. Antioxidants (Basel). 2021 Oct 13;10(10):1609 [3]. Fischer H, et al. Activation of the CFTR Cl- channel by trimethoxyflavone in vitro and in vivo. Cell Physiol Biochem. 2008;22(5-6):685-92. |
| Molecular Formula |
C18H16O5
|
|---|---|
| Molecular Weight |
312.3166
|
| Exact Mass |
312.09977361
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| CAS # |
5631-70-9
|
| Related CAS # |
5631-70-9
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| Appearance |
Solid
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| SMILES |
COC1=CC=C(C=C1)C2=CC(=O)C3=C(O2)C=C(C=C3OC)OC
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| InChi Key |
ZXJJBDHPUHUUHD-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C18H16O5/c1-20-12-6-4-11(5-7-12)15-10-14(19)18-16(22-3)8-13(21-2)9-17(18)23-15/h4-10H,1-3H3
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| Chemical Name |
5,7-dimethoxy-2-(4-methoxyphenyl)chromen-4-one
|
| Synonyms |
5,7,4'-Trimethoxyflavone
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 62~100 mg/mL (198.5~320.2 mM)
Ethanol: ~15 mg/mL (~48.0 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (8.00 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (8.00 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.2018 mL | 16.0092 mL | 32.0184 mL | |
| 5 mM | 0.6404 mL | 3.2018 mL | 6.4037 mL | |
| 10 mM | 0.3202 mL | 1.6009 mL | 3.2018 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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