| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| 250mg |
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| 500mg |
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| 1g |
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Purity: ≥98%
5-methoxyflavone, a flavonoid analog, is a novel DNA polymerase-ß inhibitor and neuroprotective agent against ß-amyloid toxicity. 5-Methoxyflavone was confirmed to be an inhibitor of DNA pol-β activity using cultured primary neurons, a valuable model for examining the neuroprotective effects of putative DNA pol-β inhibitors because treatment with Aβ causes DNA replication and cell death in these neurons. 5-methoxyflavone decreased the number of S-phase neurons and the subsequent apoptotic death induced by Aβ, which is consistent with the inhibition of DNA pol-β. 5-Methoxyflavone is the first flavonoid compound that, as opposed to having broad antioxidant and anti-inflammatory effects, is able to stop neurodegeneration through a specific molecular mechanism.
| Targets |
DNA polymerase β
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| ln Vitro |
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| ln Vivo |
5-Methoxyflavone (100, 150 mg/kg, i.p) dramatically reduces the amount of time it takes to lose the righting reflex. 5. A notable and dose-dependent decrease in spontaneous locomotor activity is shown by methylflavone (50, 100, and 150 mg/kg, i.p.). 5. The rearing response is decreased by methylflavone (50, 100 mg/kg, i.p.). 5-The grooming response was totally eliminated by methylflavone (100, 125, and 150 mg/kg, i.p. ), just like in animals treated with diazepam[3].
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| Enzyme Assay |
Human DNA Pol-β Assay [1]
DNA pol-β inhibition was evaluated with a specific DNA pol-β assay kit. The assay was carried out strictly following the manufacturer’s instructions. Briefly, a reaction mixture containing a gapped DNA template, dNTPs (both supplied with the kit), and DNA pol-β was incubated for 30 min at RT with or without the compound to be tested. The formation of repaired duplexes, which, unlike gapped DNA, selectively incorporate a fluorescent dye in the presence of Reagent U (both supplied by the kit) was determined by measuring at 535 nm with the excitation wavelength at 485 nm in a fluorimeter. OA (50 μM) was used as a positive control for inhibition. Activity was evaluated as fluorescent signal to no-enzyme-background ratio. |
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| Cell Assay |
The MTT assay is used to assess the cell viability after treating MOLT-4, U937, or PBMC cells with DMF, TMF, PMF, 5-MF, or 2'-MF at different concentrations for 24 hours.
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| Animal Protocol |
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| References |
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| Molecular Formula |
C16H12O3
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| Molecular Weight |
252.26
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| Exact Mass |
252.08
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| CAS # |
42079-78-7
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| Related CAS # |
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| Appearance |
Yellow solid powder
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| LogP |
3.5
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| tPSA |
39.440
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| SMILES |
COC1=CC=CC2=C1C(=O)C=C(O2)C3=CC=CC=C3
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| InChi Key |
XRQSPUXANRGDAV-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C16H12O3/c1-18-13-8-5-9-14-16(13)12(17)10-15(19-14)11-6-3-2-4-7-11/h2-10H,1H3
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| Chemical Name |
5-methoxy-2-phenylchromen-4-one
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| Synonyms |
5-Methoxyflavone
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
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| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 50~125 mg/mL (198.2~495.5 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (8.25 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (8.25 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (8.25 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.9642 mL | 19.8208 mL | 39.6416 mL | |
| 5 mM | 0.7928 mL | 3.9642 mL | 7.9283 mL | |
| 10 mM | 0.3964 mL | 1.9821 mL | 3.9642 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
J Nat Prod.2015 Nov 25;78(11):2704-11. |
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