| Size | Price | Stock | Qty |
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| 100mg |
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| 250mg |
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| 500mg |
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| Other Sizes |
| Targets |
DNA methyltransferases; cytidine metabolism pathways. 3'-O-Methylcytidine is a cytidine analog that inhibits DNA methyltransferases. As a modified nucleoside, it may affect RNA structure and function. The 3'-O-methyl group distinguishes it from cytidine and may affect recognition by cellular enzymes such as cytidine kinase and cytidine deaminase.
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| ln Vitro |
3'-O-Methylcytidine demonstrates activity as a cytidine nucleoside analog in cellular assays. Cytidine analogs have potential anti-metabolic and anti-tumor activities. The compound is used as a substrate in studies of nucleic acid metabolism enzymes such as cytidine kinase and cytidine deaminase.
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| ln Vivo |
In vivo activity of 3'-O-Methylcytidine has not been extensively characterized. As a cytidine analog, it would be expected to have anti-tumor activity in animal models. The compound is primarily used as a research tool for studying nucleic acid metabolism and enzyme activity.
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| Enzyme Assay |
In vitro enzyme assays for cytidine kinase or cytidine deaminase use the compound as a substrate to measure enzyme kinetics. For DNA methyltransferase inhibition, assays use recombinant DNMT enzymes and DNA substrates. 3'-O-Methylcytidine is incubated at varying concentrations, and methylation is detected by radioactivity or immunoassay.
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| Cell Assay |
Cells are cultured and treated with 3'-O-Methylcytidine at concentrations typically ranging from 1–100 μM for 24–72 hours. Cytidine analog activity is assessed by measuring effects on DNA methylation, cell proliferation, and viability. Enzyme activity is assessed in cell lysates using appropriate substrates.
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| Animal Protocol |
In vivo animal studies for 3'-O-Methylcytidine have not been reported. The compound is primarily used as an in vitro research tool. Future studies in animal models of cancer would be needed to evaluate in vivo efficacy.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for 3'-O-Methylcytidine have not been published. The compound has a molecular weight of 257.24 and formula C10H15N3O5. It is soluble in water (46 g/L at 25°C). As a cytidine analog, it is expected to be taken up by cells via nucleoside transporters.
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| Toxicity/Toxicokinetics |
Toxicology data for 3'-O-Methylcytidine have not been published. The compound is for research use only and not approved for human therapeutic applications. No systematic toxicological evaluation has been performed. Standard safety precautions should be observed.
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| References | |
| Additional Infomation |
3'-O-methylcytidine is a pyrimidine nucleoside.
3'-O-Methylcytidine (CAS: 20594-00-7, molecular formula C10H15N3O5, molecular weight 257.24) is a modified cytidine nucleoside found in RNA and in many plant and bacterial species. It is a cytidine analog that inhibits DNA methyltransferases. It is used as a substrate for studying cytidine kinase and cytidine deaminase. It is not in clinical trials. |
| Molecular Formula |
C10H15N3O5
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|---|---|
| Molecular Weight |
257.24
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| Exact Mass |
257.101
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| CAS # |
20594-00-7
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| PubChem CID |
18651938
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| Appearance |
White to off-white solid powder
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
18
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| Complexity |
397
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| Defined Atom Stereocenter Count |
4
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| SMILES |
O[C@@H]1[C@H](OC)[C@@H](CO)O[C@H]1N2C=CC(N)=NC2=O
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: ≥ 100 mg/mL (~388.7 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (9.72 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (9.72 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (9.72 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.8874 mL | 19.4371 mL | 38.8742 mL | |
| 5 mM | 0.7775 mL | 3.8874 mL | 7.7748 mL | |
| 10 mM | 0.3887 mL | 1.9437 mL | 3.8874 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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