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| Other Sizes |
| Targets |
2'-O-Methylguanosine does not have a specific therapeutic target itself but serves as a naturally occurring modified nucleoside in tRNA. The 2'-O-methyl modification at the guanosine residue in tRNA affects RNA structure, stability, and interactions with other molecules. As a modified nucleoside, it is used in research for studying RNA modification, tRNA biology, and the role of RNA modifications in gene expression. The compound is produced by tRNA guanosine-2'-O-methyltransferase.
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| ln Vitro |
The in vitro activity of 2'-O-Methylguanosine is assessed by its utility as a modified nucleoside in RNA research. It is used to study the effects of 2'-O-methylation on RNA structure and function. The compound's activity is typically assessed in biochemical assays measuring RNA stability, RNA-protein interactions, and tRNA function. As a naturally occurring modified nucleoside, it is a valuable tool for studying RNA modification biology.
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| ln Vivo |
In vivo studies for 2'-O-Methylguanosine are typically conducted in the context of studying tRNA modification and its biological functions. The compound is produced naturally in cells by tRNA guanosine-2'-O-methyltransferase. Studies in cellular and animal models are used to investigate the role of 2'-O-methylguanosine in tRNA function, protein synthesis, and disease. The compound's in vivo effects are studied through modulation of tRNA modification levels.
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| Enzyme Assay |
For tRNA modification studies, 2'-O-Methylguanosine is used as a standard for analyzing tRNA modifications by mass spectrometry or HPLC. The compound is also used in in vitro methylation assays with tRNA guanosine-2'-O-methyltransferase to study enzyme activity and substrate specificity. For RNA stability studies, RNA containing 2'-O-methylguanosine is synthesized and its stability is assessed under various conditions.
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| Cell Assay |
For cellular studies, cells are cultured and tRNA modifications are analyzed to study the role of 2'-O-methylguanosine in tRNA function. tRNA guanosine-2'-O-methyltransferase activity is assessed by measuring the incorporation of methyl groups into tRNA. The effects of 2'-O-methylguanosine on tRNA stability, aminoacylation, and ribosome binding are studied using biochemical assays. Gene expression changes resulting from altered tRNA modification are assessed by qRT-PCR and proteomics.
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| Animal Protocol |
For in vivo studies, animal models with altered tRNA guanosine-2'-O-methyltransferase expression are used to study the biological functions of 2'-O-methylguanosine. tRNA modification levels are analyzed by mass spectrometry. Phenotypic changes including growth, development, and disease susceptibility are assessed. However, detailed in vivo protocols for 2'-O-Methylguanosine as a therapeutic agent have not been extensively reported.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of 2'-O-Methylguanosine have not been fully characterized in the available literature. The compound has a molecular formula of C11H15N5O5 and a molecular weight of 297.27. It has a melting point of 227-231°C (dec.) and a density of 1.98±0.1 g/cm3 (predicted). It should be kept in a dark place, under inert atmosphere, at 2-8°C. It is soluble in water-based solvents (slightly) and DMSO (slightly).
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| Toxicity/Toxicokinetics |
2'-O-Methylguanosine is a research compound intended for laboratory use only and is not approved for human therapeutic use. As a naturally occurring modified nucleoside, it is used in research for studying RNA modification and tRNA biology. Standard laboratory safety precautions should be followed when handling this compound. Storage in a dark place, under inert atmosphere, at 2-8°C is recommended.
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| References |
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| Additional Infomation |
2'-O-methylguanosine refers to guanosine in which the hydrogen atom at the C-2' hydroxyl position is replaced by a methyl group. It is a metabolite. 2'-O-methylguanosine has been reported to exist in Saccharomyces cerevisiae, and relevant data are available for reference.
2'-O-Methylguanosine is a naturally occurring post-transcriptionally modified purine nucleoside with molecular formula C11H15N5O5 and molecular weight 297.27. It is produced in tRNAs by tRNA guanosine-2'-O-methyltransferase. The compound is used in research for studying RNA modification and tRNA biology. It is for research use only and has not been approved for clinical applications. Storage in a dark place, under inert atmosphere, at 2-8°C is recommended. |
| Molecular Formula |
C11H15N5O5
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|---|---|
| Molecular Weight |
297.27
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| Exact Mass |
297.107
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| CAS # |
2140-71-8
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| PubChem CID |
135406950
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| Appearance |
White to off-white solid powder
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| Density |
2.0±0.1 g/cm3
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| Boiling Point |
711.7ºC at 760 mmHg
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| Melting Point |
218-220ºC
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| Flash Point |
384.2ºC
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| Vapour Pressure |
2.93E-21mmHg at 25°C
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| Index of Refraction |
1.829
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| LogP |
-0.36
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
21
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| Complexity |
460
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| Defined Atom Stereocenter Count |
4
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| SMILES |
CO[C@@H]1[C@@H]([C@H](O[C@H]1N2C=NC3=C2N=C(NC3=O)N)CO)O
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| InChi Key |
OVYNGSFVYRPRCG-KQYNXXCUSA-N
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| InChi Code |
InChI=1S/C11H15N5O5/c1-20-7-6(18)4(2-17)21-10(7)16-3-13-5-8(16)14-11(12)15-9(5)19/h3-4,6-7,10,17-18H,2H2,1H3,(H3,12,14,15,19)/t4-,6-,7-,10-/m1/s1
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| Chemical Name |
2-amino-9-[(2R,3R,4R,5R)-4-hydroxy-5-(hydroxymethyl)-3-methoxyoxolan-2-yl]-1H-purin-6-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : 100 mg/mL (336.39 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (7.00 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (7.00 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (7.00 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.3639 mL | 16.8197 mL | 33.6395 mL | |
| 5 mM | 0.6728 mL | 3.3639 mL | 6.7279 mL | |
| 10 mM | 0.3364 mL | 1.6820 mL | 3.3639 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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