| Size | Price | Stock | Qty |
|---|---|---|---|
| 1g |
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| Other Sizes |
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| ln Vitro |
The production of viral structural proteins involves a partial cleavage cascade mechanism. The largest virally induced protein found in infected cells has a molecular weight of 144,000. Two polypeptides with molecular weights of 205,000 and 180,000 emerge when the infected cells are pretreated with iodoacetamide before being pulsed with 35S-methionine. When iodoacetamide is present, additional modifications take place in the viral protein precursors.[2]
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|---|---|
| ln Vivo |
2-Iodoacetamide (2.0–10 mg; 20–93 days; drinking; daily) In the secretory portion of the rat stomach, feeding invariably results in long-term, deep ulcers[2].
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| Cell Assay |
After being pretreated for 15 minutes with 2 mM iodoaeetamide, infected cells were pulsed for 30 minutes with 500 μCi of 35S-methionine. By directly comparing the corresponding autoradiogram and the stained dried slab gel, the molecular weights are determined.
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| Animal Protocol |
Animal/Disease Models: SD (Sprague-Dawley) rats[2]
Doses: 2.0-10 mg Route of Administration: Drinking water, daily, for periods of 20 to 93 days Experimental Results: Produced gastritis which was further complicated by chronic ulceration in many of the test rats . |
| References |
[1]. Peter G Hains, et al. The Impact of Commonly Used Alkylating Agents on Artifactual Peptide Modification. J Proteome Resq. 2017 Sep 1;16(9):3443-3447.
[2]. J J LALICH. Iodoacetamide induced gastric ulcers in rats. Proc Soc Exp Biol Med. 1962 Apr;109:905-8. |
| Molecular Formula |
C2H4INO
|
|---|---|
| Molecular Weight |
184.96
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| Exact Mass |
184.93
|
| CAS # |
144-48-9
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| Related CAS # |
2-Iodoacetamide-d4;1219802-64-8
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| Appearance |
Powder
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| SMILES |
C(C(=O)N)I
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| InChi Key |
PGLTVOMIXTUURA-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C2H4INO/c3-1-2(4)5/h1H2,(H2,4,5)
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| Chemical Name |
2-iodoacetamide
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| Synonyms |
Iodoacetamide; 2-Iodoacetamide
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : 40~100 mg/mL (216.3~540.7 mM)
H2O : ~100 mg/mL (~540.7 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (13.52 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (13.52 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (13.52 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. Solubility in Formulation 4: 100 mg/mL (540.66 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.4066 mL | 27.0329 mL | 54.0657 mL | |
| 5 mM | 1.0813 mL | 5.4066 mL | 10.8131 mL | |
| 10 mM | 0.5407 mL | 2.7033 mL | 5.4066 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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