| Size | Price | Stock | Qty |
|---|---|---|---|
| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
Human Endogenous Metabolite
dATP targets DNA polymerases and reverse transcriptases, serving as a direct substrate for these enzymes during DNA synthesis. The reaction mechanism involves phosphodiester bond formation, where the α-phosphate of dATP reacts with the 3'-OH group of the primer DNA strand. By providing the adenine nucleotide building block, dATP is essential for the accurate replication of genetic information. |
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| ln Vitro |
dATP demonstrates in vitro activity as a substrate for DNA polymerases in various molecular biology applications. It is essential for DNA synthesis reactions such as PCR, DNA sequencing, and molecular cloning techniques. The compound's activity is assessed by its ability to support DNA polymerization reactions in vitro, where it is incorporated into newly synthesized DNA strands opposite thymine residues in the template strand.
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| ln Vivo |
dATP is essential for DNA synthesis in vivo and is one of the four dNTPs required for DNA replication in all living organisms. It is synthesized intracellularly and its concentration is tightly regulated to maintain genomic stability. dATP is involved in DNA replication and repair, and its presence is critical for cell proliferation and viability.
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| Enzyme Assay |
dATP is used as a substrate in DNA polymerase assays to measure polymerase activity. The assay typically measures the incorporation of radiolabeled or fluorescently labeled dATP into DNA. The compound is also used in PCR, DNA sequencing, and cloning reactions, where it is added to reaction mixtures containing DNA polymerase, template DNA, primers, and other dNTPs.
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| Cell Assay |
dATP is used in cell-free in vitro assays such as PCR, DNA sequencing, and cloning. It is added to reaction mixtures containing DNA polymerase, template DNA, primers, and other dNTPs. The compound's ability to support DNA synthesis is assessed by measuring the amplification or sequencing products. It is also used in DNA labeling and other enzymatic reactions requiring nucleotide substrates.
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| Animal Protocol |
dATP is not typically administered in animal experiments as a therapeutic agent. It is a naturally occurring nucleotide that is essential for DNA synthesis in all living organisms. Its role in DNA replication makes it essential for cell proliferation and viability. In research settings, dATP may be used in cell culture media to support cell growth or in various biochemical assays.
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| ADME/Pharmacokinetics |
dATP is a naturally occurring nucleotide that is synthesized intracellularly. Its concentration is tightly regulated within cells to maintain proper DNA synthesis and genomic stability. dATP is one of the four dNTPs required for DNA synthesis. The compound is used as a research reagent in molecular biology applications. It is supplied as a trisodium salt for enhanced stability and solubility.
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| Toxicity/Toxicokinetics |
dATP is a naturally occurring nucleotide and is not considered toxic at physiological concentrations. However, imbalances in dNTP pools can lead to mutagenesis and genomic instability. The compound is intended for research use only. Standard laboratory safety precautions should be followed when handling this compound.
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| References | |
| Additional Infomation |
2'-Deoxyadenosine-5'-triphosphate trisodium (dATP trisodium) (CAS#: 54680-12-5) has the molecular formula C10H14N5O12P3·3Na and a molecular weight of 535.14. It is a purine nucleotide and critical substrate for DNA polymerases. dATP is one of the four dNTPs essential for DNA synthesis, used in PCR, DNA sequencing, and molecular cloning.
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| Molecular Formula |
C10H16N5NA3O12P3
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|---|---|
| Molecular Weight |
560.150937080383
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| Exact Mass |
513.99
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| CAS # |
54680-12-5
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| Related CAS # |
2'-Deoxyadenosine-5'-triphosphate;1927-31-7
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| PubChem CID |
156592467
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| Appearance |
White to off-white solid powder
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| Hydrogen Bond Donor Count |
6
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| Hydrogen Bond Acceptor Count |
16
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| Rotatable Bond Count |
8
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| Heavy Atom Count |
31
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| Complexity |
769
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| Defined Atom Stereocenter Count |
3
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| SMILES |
P(=O)(O)(OP(=O)(O)OP(=O)(O)O)OC[C@@H]1[C@H](C[C@H](N2C=NC3C(N)=NC=NC2=3)O1)O.[Na].[Na].[Na]
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| InChi Key |
CTWSMCPXFWEUIY-VWZUFWLJSA-N
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| InChi Code |
InChI=1S/C10H16N5O12P3.Na/c11-9-8-10(13-3-12-9)15(4-14-8)7-1-5(16)6(25-7)2-24-29(20,21)27-30(22,23)26-28(17,18)19;/h3-7,16H,1-2H2,(H,20,21)(H,22,23)(H2,11,12,13)(H2,17,18,19);/t5-,6+,7+;/m0./s1
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : 250 mg/mL (448.73 mM)
DMSO : 4.55 mg/mL (8.17 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (179.49 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.7852 mL | 8.9262 mL | 17.8524 mL | |
| 5 mM | 0.3570 mL | 1.7852 mL | 3.5705 mL | |
| 10 mM | 0.1785 mL | 0.8926 mL | 1.7852 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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