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| Targets |
(1R, 2R)-2-PCCA hydrochloride (Example 3) has an EC50 of 3 nM in cell-free assays and 603 nM in cellular experiments, making it a strong GPR88 receptor agonist[1][2]. (1R, 2R)-2-PCCA hydrochloride suppresses the GPR88-mediated cAMP synthesis via the Gαi-coupled pathway, with an EC50 of 56 nM cAMP structure in HEK293 cells that express GloSensor-22F and the human GPR88 receptor [2].
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| ln Vitro |
(1R, 2R)-2-PCCA hydrochloride (Example 3) has an EC50 of 3 nM in cell-free assays and 603 nM in cellular experiments, making it a strong GPR88 receptor agonist[1][2]. (1R, 2R)-2-PCCA hydrochloride suppresses the GPR88-mediated cAMP synthesis via the Gαi-coupled pathway, with an EC50 of 56 nM cAMP structure in HEK293 cells that express GloSensor-22F and the human GPR88 receptor [2].
In HEK293T cells transiently co-transfected with human GPR88 and a luminescent cAMP biosensor, (1R,2R)-2-PCCA (hydrochloride) inhibited isoproterenol-induced cAMP accumulation with an EC50 of 373 nM (pEC50 = 6.22). In HEK293 cells stably expressing the human GPR88 receptor and the GloSensor-22F cAMP construct, (1R,2R)-2-PCCA (hydrochloride) exhibited an EC50 of 603 nM (pEC50 = 6.22) for inhibiting isoproterenol-stimulated cAMP production. The compound did not induce calcium mobilization in HEK293T/GPR88 cells, indicating no Gαq-mediated response. [2] |
| Cell Assay |
Transient Transfection cAMP Assay: HEK293T cells were transfected with human GPR88 cDNA and a pGloSensor-22F plasmid overnight. Cells were then plated in poly-L-lysine coated 384-well plates in medium containing 1% dialyzed fetal bovine serum. After incubation, assay buffer was added, followed by the test compound at serial dilutions for 15 minutes. Luciferin and isoproterenol (200 nM final) were added, and after another 15-minute incubation, luminescence was measured to assess cAMP levels. The assay specifically measured Gαi-activation by the compound's ability to inhibit isoproterenol-stimulated cAMP production. [2]
Stable Cell Line cAMP Assay: HEK293 cells stably expressing human GPR88 and the pGloSensor-22F biosensor (GPR88-22F cells) were plated in 96-well assay plates overnight. The next day, culture medium was replaced with an equilibration medium containing GloSensor cAMP reagent for 2 hours at room temperature in the dark. Test compound dilutions were added, followed after 10 minutes by isoproterenol (100 nM final). After 30 minutes, luminescence was read. [2] Calcium Mobilization Assay (FLIPR): Calcium mobilization was measured in HEK293T/GPR88 cells using a fluorescent imaging plate reader (FLIPR) calcium assay. [2] |
| References | |
| Additional Infomation |
(1R,2R)-2-PCCA hydrochloride is a diastereomer of 2-PCCA and a potent GPR88 receptor agonist with an EC50 of 3 nM in cell-free assays and 603 nM in cellular assays.
(1R,2R)-2-PCCA (hydrochloride) is the pure (1R,2R)-diastereomer of the racemic mixture compound 2-PCCA (1). It is synthesized by asymmetric cyclopropanation using a chiral porphyrin catalyst, followed by coupling and deprotection steps. Its chemical name is (1R,2R)-2-(pyridin-2-yl)cyclopropanecarboxylic acid [(2S,3S)-2-amino-3-methylpentyl]-(4'-propylbiphenyl-4-yl)amide dihydrochloride. In transient transfection cAMP assays, its potency was approximately 5 times that of its (1S,2S)-diastereform (3). This study suggests that GPR88 is a promising drug target for basal ganglia-related diseases, and (1R,2R)-2-PCCA (hydrochloride) can serve as a pharmacological tool for studying GPR88 function. [2] |
| Molecular Formula |
C30H39CL2N3O
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|---|---|
| Molecular Weight |
528.556165933609
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| Exact Mass |
527.247
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| CAS # |
1609563-71-4
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| Related CAS # |
2-PCCA hydrochloride
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| PubChem CID |
134128291
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| Appearance |
Light yellow to khaki solid powder
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
10
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| Heavy Atom Count |
36
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| Complexity |
623
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| Defined Atom Stereocenter Count |
4
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| SMILES |
Cl.Cl.O=C([C@@H]1C[C@H]1C1C=CC=CN=1)N(C1C=CC(C2C=CC(CCC)=CC=2)=CC=1)C[C@H]([C@@H](C)CC)N
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| InChi Key |
ZRMICUFWAJDIQJ-QUDNCWCRSA-N
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| InChi Code |
InChI=1S/C30H37N3O.2ClH/c1-4-8-22-10-12-23(13-11-22)24-14-16-25(17-15-24)33(20-28(31)21(3)5-2)30(34)27-19-26(27)29-9-6-7-18-32-29;;/h6-7,9-18,21,26-28H,4-5,8,19-20,31H2,1-3H3;2*1H/t21-,26+,27+,28+;;/m0../s1
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| Chemical Name |
(1R,2R)-N-[(2S,3S)-2-amino-3-methylpentyl]-N-[4-(4-propylphenyl)phenyl]-2-pyridin-2-ylcyclopropane-1-carboxamide;dihydrochloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~94.60 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (4.73 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (4.73 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.8919 mL | 9.4597 mL | 18.9193 mL | |
| 5 mM | 0.3784 mL | 1.8919 mL | 3.7839 mL | |
| 10 mM | 0.1892 mL | 0.9460 mL | 1.8919 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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