| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 100mg | |||
| Other Sizes |
| Targets |
Zinquin ethyl ester targets zinc ions (Zn²⁺) in cells and tissues. It binds to zinc with high affinity and selectivity, producing a fluorescent signal that can be detected by fluorescence microscopy or flow cytometry. The compound is used to measure intracellular zinc levels and to study zinc dynamics in living cells.
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|---|---|
| ln Vitro |
In vitro, Zinquin ethyl ester is used to measure zinc concentrations in cell lysates, culture media, and biological fluids. It is also used in cell-based assays to monitor changes in intracellular zinc levels in response to stimuli or treatments. The compound's fluorescence is proportional to the zinc concentration, allowing for quantitative measurements.
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| ln Vivo |
In vivo, Zinquin ethyl ester is used in animal models to study zinc distribution and dynamics in tissues. The compound can be administered to animals, and tissues can be examined for zinc-associated fluorescence. It has been used in studies of zinc in the brain, pancreas, and other organs.
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| Enzyme Assay |
The in vitro assay for Zinquin ethyl ester involves incubating the compound with a sample (e.g., cell lysate, buffer) and measuring fluorescence at excitation/emission wavelengths appropriate for the probe. A standard curve is prepared using known concentrations of zinc to quantify the zinc concentration in the sample.
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| Cell Assay |
For in vitro cellular assays, cells are loaded with Zinquin ethyl ester by incubating with the compound in culture medium. After washing, cells are stimulated or treated as desired, and fluorescence is measured using a fluorescence plate reader, flow cytometry, or fluorescence microscopy. Changes in fluorescence intensity reflect changes in intracellular zinc levels.
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| Animal Protocol |
For in vivo studies, Zinquin ethyl ester is administered to animals via injection (e.g., intraperitoneal or intravenous). After a suitable period for distribution, tissues are harvested and examined for fluorescence. Alternatively, the compound can be used in live animal imaging to study zinc dynamics in real time.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for Zinquin ethyl ester are limited. The compound is cell-permeable and distributes into tissues.
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| Toxicity/Toxicokinetics |
The toxicology of Zinquin ethyl ester is related to its use as a fluorescent probe. At the low concentrations used for imaging, it is generally considered non-toxic. However, standard laboratory safety precautions should be followed.
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| References | |
| Additional Infomation |
Zinquin ethyl ester is a fluorescent probe for detecting and quantifying zinc ions in biological systems. It is a research tool for studying zinc homeostasis, zinc signaling, and the role of zinc in health and disease. The compound is not approved for human use and is intended for research purposes only.
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| Molecular Formula |
C21H22N2O5S
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|---|---|
| Molecular Weight |
414.47478
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| Exact Mass |
414.124
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| CAS # |
181530-09-6
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| PubChem CID |
3638243
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| Appearance |
White to off-white solid powder
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| Density |
1.3±0.1 g/cm3
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| Boiling Point |
578.0±60.0 °C at 760 mmHg
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| Flash Point |
303.3±32.9 °C
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| Vapour Pressure |
0.0±1.6 mmHg at 25°C
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| Index of Refraction |
1.619
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| LogP |
5.08
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
8
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| Heavy Atom Count |
29
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| Complexity |
642
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
KCASTCXJTDRDFT-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C21H22N2O5S/c1-4-27-20(24)13-28-17-11-16-8-7-15(3)22-21(16)19(12-17)23-29(25,26)18-9-5-14(2)6-10-18/h5-12,23H,4,13H2,1-3H3
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| Chemical Name |
ethyl 2-[2-methyl-8-[(4-methylphenyl)sulfonylamino]quinolin-6-yl]oxyacetate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ≥ 50 mg/mL (~120.64 mM)
H2O : < 0.1 mg/mL |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 5 mg/mL (12.06 mM) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 50.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.4127 mL | 12.0636 mL | 24.1272 mL | |
| 5 mM | 0.4825 mL | 2.4127 mL | 4.8254 mL | |
| 10 mM | 0.2413 mL | 1.2064 mL | 2.4127 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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