| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
|
||
| 10mg |
|
||
| 100mg |
|
||
| 250mg | |||
| Other Sizes |
| Targets |
- `Xylotriose` is the natural substrate of beta-xylosidase (β-xylosidase), an enzyme that catalyzes the hydrolysis of xylooligosaccharides to xylose; the Michaelis constant (Km) of β-xylosidase for `Xylotriose` is 1.2 mM [1]
|
|---|---|
| ln Vitro |
- In the beta-xylosidase activity assay using `Xylotriose` as substrate, 0.5-5 mM `Xylotriose` showed a dose-dependent increase in hydrolysis rate: 0.5 mM `Xylotriose` resulted in a reaction rate of 0.08 μmol·min⁻¹·mg⁻¹ (enzyme protein), 2 mM resulted in 0.21 μmol·min⁻¹·mg⁻¹, and 5 mM resulted in 0.32 μmol·min⁻¹·mg⁻¹ (saturated rate). The hydrolysis products were identified as xylose (major, ~70%) and xylobiose (minor, ~30%) via high-performance liquid chromatography (HPLC) [1]
- In the enzyme-coupled assay (beta-xylosidase + glucose dehydrogenase), `Xylotriose` (1 mM) was completely hydrolyzed by beta-xylosidase within 30 minutes at 37°C, and the generated xylose was further converted to xylono-1,5-lactone, accompanied by a linear increase in NADH absorbance at 340 nm (detection range: 0.01-5 mM `Xylotriose`) [1] |
| Enzyme Assay |
- Beta-xylosidase activity assay with `Xylotriose` as substrate: The reaction system (total volume 1 mL) contained 50 mM sodium acetate buffer (pH 5.0), 0.1-5 mM `Xylotriose`, and 0.02 mg/mL purified beta-xylosidase. The mixture was incubated at 37°C for 10-60 minutes, then the reaction was terminated by adding 0.2 mL of 1 M Tris-HCl buffer (pH 8.5). The amount of xylose (hydrolysis product) was quantified by the glucose dehydrogenase-coupled method: add 0.5 mL of 0.1 M Tris-HCl (pH 8.0), 0.1 mL of 5 mM NAD⁺, and 0.01 mg/mL glucose dehydrogenase, incubate at 30°C for 20 minutes, then measure absorbance at 340 nm. The enzyme activity was calculated based on the NADH standard curve [1]
- HPLC analysis of `Xylotriose` hydrolysis products: After the enzyme reaction, 0.5 mL of the reaction mixture was filtered through a 0.22 μm membrane. The filtrate was injected into an HPLC system equipped with a carbohydrate analysis column. The mobile phase was acetonitrile-water (75:25, v/v) at a flow rate of 1 mL/min, and detection was performed using a refractive index detector. Xylose, xylobiose, and `Xylotriose` were identified by comparing retention times with standard compounds [1] |
| References | |
| Additional Infomation |
β-D-Xylp-(1->4)-β-D-Xylp-(1->4)-D-Xylp is a xylotriose composed of three D-xylose units linked by β-(1->4) glycosidic bonds.
Xylotriose has been reported to exist in Streptomyces rameus, and relevant data are available. - Xylotriose is a xylo-oligosaccharide composed of three xylose units linked by β-1,4-glycosidic bonds, and is a natural degradation product of xylan (the main component of plant cell walls) [1]. - The enzyme-coupled assay developed in the literature using xylotriose as a substrate has high specificity for β-xylosidase: when xylotriose is used as a substrate, no cross-reaction with other glycosidases (such as α-glucosidase, β-glucosidase) was observed. Xylitol was used as the sole substrate [1] - Xylitol was more stable than xylobiose in aqueous solution (4°C, pH 5.0): after 7 days of storage, xylitol retained 95% of its initial concentration, while xylobiose retained only 82% [1] |
| Molecular Formula |
C15H26O13
|
|---|---|
| Molecular Weight |
414.3591
|
| Exact Mass |
414.137
|
| CAS # |
47592-59-6
|
| PubChem CID |
10201852
|
| Appearance |
White to off-white solid powder
|
| LogP |
-5.6
|
| Hydrogen Bond Donor Count |
8
|
| Hydrogen Bond Acceptor Count |
13
|
| Rotatable Bond Count |
4
|
| Heavy Atom Count |
28
|
| Complexity |
508
|
| Defined Atom Stereocenter Count |
11
|
| SMILES |
C1[C@H]([C@@H]([C@H]([C@@H](O1)O[C@@H]2CO[C@H]([C@@H]([C@H]2O)O)O[C@@H]3COC([C@@H]([C@H]3O)O)O)O)O)O
|
| InChi Key |
JCSJTDYCNQHPRJ-FDVJSPBESA-N
|
| InChi Code |
InChI=1S/C15H26O13/c16-4-1-25-14(11(21)7(4)17)28-6-3-26-15(12(22)9(6)19)27-5-2-24-13(23)10(20)8(5)18/h4-23H,1-3H2/t4-,5-,6-,7+,8+,9+,10-,11-,12-,13?,14+,15+/m1/s1
|
| Chemical Name |
(2S,3R,4S,5R)-2-[(3R,4R,5R,6S)-4,5-dihydroxy-6-[(3R,4R,5R)-4,5,6-trihydroxyoxan-3-yl]oxyoxan-3-yl]oxyoxane-3,4,5-triol
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O : ~125 mg/mL (~301.67 mM)
DMSO : ~100 mg/mL (~241.34 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (6.03 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (6.03 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (6.03 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. Solubility in Formulation 4: 50 mg/mL (120.67 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.4134 mL | 12.0668 mL | 24.1336 mL | |
| 5 mM | 0.4827 mL | 2.4134 mL | 4.8267 mL | |
| 10 mM | 0.2413 mL | 1.2067 mL | 2.4134 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
