| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| 25mg |
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| 100mg | |||
| Other Sizes |
| ln Vitro |
- Xylohexaose was used as a substrate to evaluate the hydrolytic activity of a hybrid xylanase (XynA1-A2) and its parental xylanases (XynA1 and XynA2). After incubation at 50°C (pH 6.0) for 24 h, XynA1-A2 hydrolyzed Xylohexaose to produce xylose (X1, ~35%), xylobiose (X2, ~40%), xylotriose (X3, ~20%), and trace amounts of xylotetraose (X4); XynA1 produced more X1 (~45%) and X2 (~35%), while XynA2 produced more X3 (~30%) and X4 (~15%) [1]
- Xylohexaose was hydrolyzed by a purified xylanase from Aspergillus flavus (AfXyn) at optimal conditions (55°C, pH 5.0). After 12 h of reaction, the hydrolysis rate of Xylohexaose (10 mg/mL) by AfXyn (0.5 U/mL) reached ~92%, with the main products being X2 (~50%) and X3 (~40%), and a small amount of X1 (~10%) [2] |
|---|---|
| Enzyme Assay |
- For hydrolytic activity assay of hybrid xylanase and parental enzymes (Reference [1]): The reaction mixture contained 50 mM sodium phosphate buffer (pH 6.0), 5 mg/mL Xylohexaose, and 0.2 U/mL xylanase (XynA1-A2, XynA1, or XynA2). The mixture was incubated at 50°C for 24 h, and the reaction was terminated by boiling for 10 minutes. The hydrolytic products were analyzed by high-performance liquid chromatography (HPLC) using a carbohydrate analysis column. The mobile phase was acetonitrile-water (75:25, v/v) at a flow rate of 1.0 mL/min, and the column temperature was 30°C. Product concentrations were quantified by comparing peak areas with standard curves of X1, X2, X3, and X4 [1]
- For hydrolytic activity assay of Aspergillus flavus xylanase (Reference [2]): The reaction system included 20 mM citrate-phosphate buffer (pH 5.0), 10 mg/mL Xylohexaose, and 0.5 U/mL AfXyn. The mixture was incubated at 55°C, and samples were taken at 2-h intervals for 12 h. Each sample was boiled for 5 minutes to stop the reaction, then centrifuged at 12,000 × g for 10 minutes. The supernatant was filtered through a 0.22 μm membrane and analyzed by HPLC (carbohydrate column). The mobile phase was 80% acetonitrile (v/v) with a flow rate of 0.8 mL/min, and detection was performed using a refractive index detector. The hydrolysis rate was calculated based on the reduction of Xylohexaose concentration, and product composition was determined by peak identification and quantification [2] |
| References |
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| Additional Infomation |
Xylan hexasaccharide (X6) is a xylo-hexasaccharide oligosaccharide and a specific substrate for xylanases. It is widely used to evaluate the hydrolytic efficiency and product specificity of xylanases [1, 2]. In reference [1], xylan hexasaccharide was used to compare the catalytic properties of hybrid xylanases and their parents. The results showed that the hybrid enzymes inherited the high hydrolytic efficiency of XynA1 and the product specificity of XynA2 [1]. In reference [2], xylan hexasaccharide helped to confirm the excellent stability of AfXyn: after pre-incubation at 60°C for 2 hours, the hydrolytic activity of AfXyn on xylan hexasaccharide remained above 85%, indicating that it has good thermal stability [2].
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| Molecular Formula |
C30H50O25
|
|---|---|
| Molecular Weight |
810.7030
|
| Exact Mass |
810.264
|
| CAS # |
49694-21-5
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| PubChem CID |
74539951
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| Appearance |
White to off-white solid powder
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| Density |
1.77 g/cm3
|
| Boiling Point |
1142.7±65.0℃ at 760 mmHg
|
| Melting Point |
236-237℃
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| LogP |
-10.2
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| Hydrogen Bond Donor Count |
14
|
| Hydrogen Bond Acceptor Count |
25
|
| Rotatable Bond Count |
10
|
| Heavy Atom Count |
55
|
| Complexity |
1210
|
| Defined Atom Stereocenter Count |
23
|
| SMILES |
C1[C@H]([C@@H]([C@H]([C@@H](O1)O[C@@H]2CO[C@H]([C@@H]([C@H]2O)O)O[C@@H]3CO[C@H]([C@@H]([C@H]3O)O)O[C@@H]4CO[C@H]([C@@H]([C@H]4O)O)O[C@@H]5CO[C@H]([C@@H]([C@H]5O)O)O[C@@H]6COC([C@@H]([C@H]6O)O)O)O)O)O
|
| InChi Key |
FTTUBRHJNAGMKL-HWHAXOAESA-N
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| InChi Code |
InChI=1S/C30H50O25/c31-7-1-46-26(20(39)13(7)32)52-9-3-48-28(22(41)15(9)34)54-11-5-50-30(24(43)17(11)36)55-12-6-49-29(23(42)18(12)37)53-10-4-47-27(21(40)16(10)35)51-8-2-45-25(44)19(38)14(8)33/h7-44H,1-6H2/t7-,8-,9-,10-,11-,12-,13+,14+,15+,16+,17+,18+,19-,20-,21-,22-,23-,24-,25?,26+,27+,28+,29+,30+/m1/s1
|
| Chemical Name |
(2S,3R,4S,5R)-2-[(3R,4R,5R,6S)-6-[(3R,4R,5R,6S)-6-[(3R,4R,5R,6S)-6-[(3R,4R,5R,6S)-4,5-dihydroxy-6-[(3R,4R,5R)-4,5,6-trihydroxyoxan-3-yl]oxyoxan-3-yl]oxy-4,5-dihydroxyoxan-3-yl]oxy-4,5-dihydroxyoxan-3-yl]oxy-4,5-dihydroxyoxan-3-yl]oxyoxane-3,4,5-triol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O : ~400 mg/mL (~493.40 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (123.35 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.2335 mL | 6.1675 mL | 12.3350 mL | |
| 5 mM | 0.2467 mL | 1.2335 mL | 2.4670 mL | |
| 10 mM | 0.1234 mL | 0.6168 mL | 1.2335 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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