| Size | Price | Stock | Qty |
|---|---|---|---|
| 50mg |
|
||
| 100mg |
|
||
| 250mg |
|
||
| 500mg |
|
||
| Other Sizes |
| ln Vitro |
Advice (This is our suggested protocol, which should be adjusted to suit your particular circumstances as it simply offers guidance). 1. 20 mg of X-Gluc dicyclohexylamine should be dissolved in 1 mL of dimethylformamide (DMF) to create the X-Gluc master mix. 2. Fill the agar medium plate with the prepared X-Gluc solution. 50 μg/mL is the final concentration. Sterilization is not necessary. 3. When using plates for organism inoculation, let them air dry. 4. After inoculation, incubate the plate for 16–24 hours at 35°C.
X-GLUC was evaluated as a chromogenic substrate for detecting β-glucuronidase activity, which is a marker for Escherichia coli. When incorporated at a concentration of 50 μg/mL into Peptone-Tergitol Agar (PTA) medium, it allowed for the direct enumeration of E. coli colonies after 24 hours of incubation at 35°C. β-Glucuronidase-positive E. coli colonies hydrolyze X-GLUC to produce an insoluble blue precipitate (indigo derivative), localizing the color within the colony. In recovery tests using artificially inoculated raw minced chicken, the enumeration of two E. coli strains on PTX agar (containing X-GLUC) was comparable to that on PTG agar (containing the fluorogenic substrate MUG) and to counts on non-selective Plate Count Agar. The substrate showed no discernible inhibitory effect on colony size or number at the tested concentration. However, X-GLUC was found to be unsuitable as an indicator in liquid broth media (e.g., Lauryl Sulfate Broth) due to insufficient color development within 24 hours. [1] |
|---|---|
| References | |
| Additional Infomation |
5-Bromo-4-chloro-3-indolyl-β-D-glucuronide (X-GLUC) is a chromogenic substrate for the detection of β-glucuronidase. This study evaluated X-GLUC as an alternative to the fluorescent substrate 4-methylumbelliferone-β-D-glucuronide (MUG) for a 24-hour direct plate count of E. coli. The main advantages of X-GLUC compared to MUG are: (a) colony counting does not require UV irradiation; and (b) the blue reaction is limited to positive colonies, avoiding difficulties in colony identification due to fluorescence diffusion. Its main disadvantage is its high cost (approximately 25 times that of MUG), limiting its practicality for routine use in plate media. The substrate must be dissolved in dimethylformamide (DMF) before being added to the plate. (The text then mentions treated agar.) The study concluded that, under the test conditions, X-GLUC in agar medium at a concentration of 50 μg/mL was as effective as MUG in recovering Escherichia coli from artificially inoculated food samples. [1]
|
| Molecular Formula |
C14H14NO7BR.C6H13N
|
|---|---|
| Molecular Weight |
487.34158
|
| CAS # |
18656-96-7
|
| Related CAS # |
X-Gluc cyclohexanamine;114162-64-0;X-Gluc sodium;129541-41-9
|
| Appearance |
White to off-white solid powder
|
| Boiling Point |
736.7ºC at 760mmHg
|
| Melting Point |
249 °C
|
| Flash Point |
399.3ºC
|
| Vapour Pressure |
8.69E-23mmHg at 25°C
|
| LogP |
0.081
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ≥ 30 mg/mL (~49.67 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (4.14 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (4.14 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (4.14 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.0520 mL | 10.2598 mL | 20.5196 mL | |
| 5 mM | 0.4104 mL | 2.0520 mL | 4.1039 mL | |
| 10 mM | 0.2052 mL | 1.0260 mL | 2.0520 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
