| Size | Price | Stock | Qty |
|---|---|---|---|
| 10mg |
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| 25mg |
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| 50mg | |||
| 100mg | |||
| 250mg | |||
| 500mg | |||
| 1g | |||
| Other Sizes |
| Targets |
WST-3 targets NADH (reduced nicotinamide adenine dinucleotide) as a substrate for reduction. In the presence of an electron mediator, it is reduced to a water-soluble formazan product. Unlike traditional tetrazolium salts such as MTT, WST-3 produces a water-soluble formazan that does not require solubilization steps, making it more convenient for high-throughput assays.
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|---|---|
| ln Vitro |
In vitro, WST-3 is a highly sensitive tetrazolium reagent (light red) that produces a water-soluble formazan (dark red color) upon reduction. The WST-3 formazan has an absorption maximum at 433 nm with a molar extinction coefficient (ε) of 3.1 × 10⁴. The compound is used in colorimetric assays to quantify cellular metabolic activity and cell number.
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| ln Vivo |
In vivo activity data for WST-3 are not applicable, as it is a laboratory reagent used for in vitro assays rather than a therapeutic compound. The compound is not intended for in vivo administration and is used exclusively as a research tool in cell biology and biochemistry applications.
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| Enzyme Assay |
In vitro enzyme assays for WST-3 involve measuring its reduction by NADH or NADPH in the presence of an electron mediator such as 1-methoxy PMS. The reaction is monitored spectrophotometrically by measuring the absorbance of the formazan product at 433 nm. The rate of reduction is proportional to the concentration of the reducing agent or enzyme activity.
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| Cell Assay |
In vitro cellular assays for WST-3 are performed using cultured cells. Cells are incubated with WST-3 and an electron mediator. Viable cells with active mitochondrial dehydrogenases reduce WST-3 to a water-soluble formazan product. The absorbance of the formazan at 433 nm is measured and is proportional to the number of viable cells. The assay is used for cell viability, proliferation, and cytotoxicity testing.
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| Animal Protocol |
In vivo animal experiments are not applicable for WST-3, as it is a laboratory reagent used for in vitro assays rather than a therapeutic compound. The compound is used exclusively in cell culture and biochemical applications and is not administered to animals for efficacy or safety testing.
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| ADME/Pharmacokinetics |
WST-3 has a molecular weight of 696.34 and molecular formula C₁₉H₁₀IN₆NaO₁₀S₂. Its chemical name is 3-(2,4-dinitrophenyl)-5-(2,4-disulfophenyl)-2-(4-iodophenyl)-2H-tetrazolium inner salt sodium salt. It is a crystalline solid soluble in water at 10 mg/mL with λmax at 233 nm. It should be stored at -20°C.
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| Toxicity/Toxicokinetics |
Toxicological data for WST-3 are not applicable, as it is a laboratory reagent rather than a therapeutic compound. The compound is used in research applications at low concentrations in cell culture assays. Standard laboratory safety precautions should be followed when handling this reagent.
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| References |
: Wawra A, Friesl-Hanl W, Jäger A, Puschenreiter M, Soja G, Reichenauer T, Watzinger A. Investigations of microbial degradation of polycyclic aromatic hydrocarbons based on (13)C-labeled phenanthrene in a soil co-contaminated with trace elements using a plant assisted approach. Environ Sci Pollut Res Int. 2018 Mar;25(7):6364-6377.
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| Additional Infomation |
WST-3 is a water-soluble tetrazolium dye also known as Water-Soluble Tetrazolium 3. It is used as a highly sensitive reagent for colorimetric cell viability assays. Unlike MTT, WST-3 produces a water-soluble formazan product that does not require solubilization, making it ideal for high-throughput screening applications. This product is for research use only.
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| Molecular Formula |
C19H10IN6NAO10S2
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|---|---|
| Molecular Weight |
696.3411
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| Exact Mass |
695.884
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| CAS # |
515111-36-1
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| PubChem CID |
9810247
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| Appearance |
Light yellow to green yellow solid powder
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| LogP |
4.648
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
12
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
39
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| Complexity |
1070
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
LKVNWZJLLRMMAS-UHFFFAOYSA-M
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| InChi Code |
InChI=1S/C19H11IN6O10S2.Na/c20-11-1-3-12(4-2-11)23-21-19(15-7-6-14(37(31,32)33)10-18(15)38(34,35)36)22-24(23)16-8-5-13(25(27)28)9-17(16)26(29)30/h1-10H,(H-,31,32,33,34,35,36)/q+1/p-1
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| Chemical Name |
Sodium 4-[2-(2,4-dinitrophenyl)-3-(4-iodophenyl)tetrazol-2-ium-5-yl]benzene-1,3-disulfonate
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| Synonyms |
WST-3 WST 3 WST3
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~143.61 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (3.59 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.4361 mL | 7.1804 mL | 14.3608 mL | |
| 5 mM | 0.2872 mL | 1.4361 mL | 2.8722 mL | |
| 10 mM | 0.1436 mL | 0.7180 mL | 1.4361 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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