| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| Other Sizes |
| ln Vivo |
- Insecticidal activity against Mythimna separata 3rd-instar larvae: Wilforgine caused toxic effects, with 65% larval mortality at 20 μg/mL after 48 hours (vs. 20% in control). Surviving larvae had 40% reduced body weight vs. control. It damaged muscle microstructure (disrupted fiber arrangement) and ultrastructure (swollen mitochondria, broken myofibrils, increased vacuolation) [2]
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| Animal Protocol |
- Mythimna separata treatment: 3rd-instar larvae were reared at 25±1°C (16L:8D, 70±5% RH). Wilforgine was dissolved in distilled water to 5/10/20 μg/mL; maize leaves were soaked in solutions for 30s, air-dried, and fed to larvae (10 larvae/group, 3 replicates; control: distilled water-soaked leaves). Mortality and body weight were recorded at 24/48h. Thoracic muscle from survivors was fixed, dehydrated, embedded, sectioned, stained, and observed via light/transmission electron microscope [2]
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| References |
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| Additional Infomation |
It has been reported that both Tripterygium wilfordii and Tripterygium hypoglaucum contain Wilforgine, and there is relevant data. Wilforgine is a sesquiterpene alkaloid derived from Tripterygium wilfordii Hook. F. [1] HPLC quantitative analysis of Wilforgine in Tripterygium wilfordii extract: C18 column, mobile phase methanol-water (75:25, v/v), flow rate 1.0 mL/min, detection wavelength 220 nm, column temperature 30°C. Linear range: 0.05–2.0 μg (r=0.9998) [1]
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| Molecular Formula |
C41H47NO19
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|---|---|
| Molecular Weight |
857.8072
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| Exact Mass |
857.274
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| CAS # |
37239-47-7
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| PubChem CID |
124030
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| Appearance |
White to off-white solid powder
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| Density |
1.4±0.1 g/cm3
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| Boiling Point |
859.9±65.0 °C at 760 mmHg
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| Melting Point |
211℃
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| Flash Point |
473.8±34.3 °C
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| Vapour Pressure |
0.0±0.3 mmHg at 25°C
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| Index of Refraction |
1.583
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| LogP |
5.32
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
20
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| Rotatable Bond Count |
14
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| Heavy Atom Count |
61
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| Complexity |
1790
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| Defined Atom Stereocenter Count |
10
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| SMILES |
CC1CCC2=C(C=CC=N2)C(=O)OC[C@]3([C@@H]4[C@H]([C@H]([C@@]5([C@H]([C@H]([C@@H](C([C@]5([C@@H]4OC(=O)C)O3)(C)O)OC1=O)OC(=O)C6=COC=C6)OC(=O)C)COC(=O)C)OC(=O)C)OC(=O)C)C
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| InChi Key |
QFIYSPKZWOALMZ-YHQLYFKISA-N
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| InChi Code |
InChI=1S/C41H47NO19/c1-19-11-12-27-26(10-9-14-42-27)37(50)54-17-38(7)28-29(55-21(3)44)33(57-23(5)46)40(18-53-20(2)43)34(58-24(6)47)30(59-36(49)25-13-15-52-16-25)32(60-35(19)48)39(8,51)41(40,61-38)31(28)56-22(4)45/h9-10,13-16,19,28-34,51H,11-12,17-18H2,1-8H3/t19?,28-,29-,30+,31-,32+,33-,34+,38+,39?,40-,41+/m1/s1
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| Chemical Name |
[(1S,3R,18S,19R,20R,21R,22S,23R,24R,25R)-20,22,23,25-tetraacetyloxy-21-(acetyloxymethyl)-26-hydroxy-3,15,26-trimethyl-6,16-dioxo-2,5,17-trioxa-11-azapentacyclo[16.7.1.01,21.03,24.07,12]hexacosa-7(12),8,10-trien-19-yl] furan-3-carboxylate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~116.58 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (2.91 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (2.91 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.1658 mL | 5.8288 mL | 11.6576 mL | |
| 5 mM | 0.2332 mL | 1.1658 mL | 2.3315 mL | |
| 10 mM | 0.1166 mL | 0.5829 mL | 1.1658 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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