| Size | Price | Stock | Qty |
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| 25mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
Vitexin-2-O-rhamnoside targets oxidative stress pathways, protecting cells against H₂O₂-mediated oxidative damage. As a flavonoid glycoside, it exhibits antioxidant properties by scavenging free radicals and reducing oxidative stress. The compound may also modulate signaling pathways involved in cell survival and apoptosis. Its cardioprotective effects are likely mediated through its antioxidant activity and potential effects on vascular function.
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| ln Vitro |
In vitro studies have demonstrated that Vitexin-2-O-rhamnoside protects against H₂O₂-mediated oxidative stress damage. The compound exhibits antioxidant properties and can be safely used for a wide range of concentrations. Its ability to protect cells from oxidative damage makes it a valuable tool for studying oxidative stress and cytoprotection. The compound's antioxidant activity is attributed to its flavonoid structure, which can scavenge free radicals and chelate metal ions.
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| ln Vivo |
In vivo, Vitexin-2-O-rhamnoside is a component of hawthorn (Crataegus pinnatifida) leaf and flower extracts, which have been used in traditional medicine for cardiovascular health. The compound may contribute to the cardioprotective effects of hawthorn through its antioxidant activity and potential effects on vascular function. However, specific in vivo studies focusing exclusively on Vitexin-2-O-rhamnoside are limited.
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| Enzyme Assay |
In vitro non-cell assays for Vitexin-2-O-rhamnoside typically involve antioxidant activity measurements using DPPH, ABTS, or FRAP assays. The compound is incubated with the radical-generating system, and the decrease in absorbance is measured spectrophotometrically to calculate the scavenging activity and IC₅₀ values. These assays provide a quantitative measure of the compound's antioxidant capacity.
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| Cell Assay |
In vitro cell-based assays for Vitexin-2-O-rhamnoside use cell lines such as endothelial cells, cardiomyocytes, or neuronal cells exposed to H₂O₂ or other oxidative insults. Cells are treated with the compound at various concentrations, and parameters such as cell viability (MTT or CCK-8 assays), reactive oxygen species levels (fluorescent probes such as DCFH-DA), and markers of oxidative damage (MDA, protein carbonyls) are measured to evaluate cytoprotective effects.
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| Animal Protocol |
In vivo animal studies for Vitexin-2-O-rhamnoside are limited. Standard protocols for evaluating cardioprotective compounds would involve oral administration of the compound to rodent models of ischemia-reperfusion injury, hypertension, or oxidative stress. Parameters assessed would include infarct size, cardiac function, oxidative stress markers, and histopathological examination of cardiac tissues. However, specific published in vivo data for this compound are limited.
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| ADME/Pharmacokinetics |
Vitexin-2-O-rhamnoside has a molecular weight of 578.52 g/mol and a molecular formula of C₂₇H₃₀O₁₄. It is also known as Vitexin 2''-O-rhamnoside. The compound is a flavonoid glycoside found in hawthorn leaves. It should be stored under appropriate conditions as recommended by the manufacturer. Detailed pharmacokinetic parameters have not been extensively characterized.
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| Toxicity/Toxicokinetics |
Vitexin-2-O-rhamnoside is considered to have low toxicity based on its natural occurrence in hawthorn and its safe use at a wide range of concentrations. The compound is classified as a research reagent and is not intended for human therapeutic use. Standard laboratory safety precautions should be followed when handling the compound. Comprehensive toxicological studies have not been performed.
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| References | |
| Additional Infomation |
Vitexin 2''-O-α-L-rhamnoside is a derivative of vitexin, with an α-L-rhamnosine residue linked to the 2'' position of its glucol moiety. It is a plant metabolite. It is a C-glycoside compound, a trihydroxyflavonoid, and a disaccharide derivative. It is functionally related to vitexin. It is the conjugate acid of vitexin 2''-O-α-L-rhamnoside (1-). Vitexin 2''-O-rhamnoside has been reported in tea (Camellia sinensis), soybean (Glycine max), and other organisms with relevant data. See also: Top (part) of the inflorescence of Crataegus monogyna.
Vitexin-2-O-rhamnoside is a flavonoid glycoside found in the leaves of Crataegus pinnatifida (hawthorn). It is also known as Vitexin 2''-O-rhamnoside. The compound protects against H₂O₂-mediated oxidative stress damage and has antioxidant properties. It is used as a research tool for studying oxidative stress and cardioprotection. Not approved for clinical use; intended for research purposes only. |
| Molecular Formula |
C27H30O14
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|---|---|
| Molecular Weight |
578.5187
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| Exact Mass |
578.163
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| CAS # |
64820-99-1
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| PubChem CID |
5282151
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| Appearance |
White to yellow solid powder
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| Density |
1.7±0.1 g/cm3
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| Boiling Point |
898.8±65.0 °C at 760 mmHg
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| Melting Point |
215ºC
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| Flash Point |
299.5±27.8 °C
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| Vapour Pressure |
0.0±0.3 mmHg at 25°C
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| Index of Refraction |
1.751
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| LogP |
1.86
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| Hydrogen Bond Donor Count |
9
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| Hydrogen Bond Acceptor Count |
14
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
41
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| Complexity |
954
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| Defined Atom Stereocenter Count |
10
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| SMILES |
C[C@H]1[C@@H]([C@H]([C@H]([C@@H](O1)O[C@@H]2[C@H]([C@@H]([C@H](O[C@H]2C3=C(C=C(C4=C3OC(=CC4=O)C5=CC=C(C=C5)O)O)O)CO)O)O)O)O)O
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| InChi Key |
LYGPBZVKGHHTIE-HUBYJIGHSA-N
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| InChi Code |
InChI=1S/C27H30O14/c1-9-19(33)21(35)23(37)27(38-9)41-26-22(36)20(34)16(8-28)40-25(26)18-13(31)6-12(30)17-14(32)7-15(39-24(17)18)10-2-4-11(29)5-3-10/h2-7,9,16,19-23,25-31,33-37H,8H2,1H3/t9-,16+,19-,20+,21+,22-,23+,25-,26+,27-/m0/s1
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| Chemical Name |
8-[(2S,3R,4S,5S,6R)-4,5-dihydroxy-6-(hydroxymethyl)-3-[(2S,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methyloxan-2-yl]oxyoxan-2-yl]-5,7-dihydroxy-2-(4-hydroxyphenyl)chromen-4-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~172.85 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (4.32 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (4.32 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.7285 mL | 8.6427 mL | 17.2855 mL | |
| 5 mM | 0.3457 mL | 1.7285 mL | 3.4571 mL | |
| 10 mM | 0.1729 mL | 0.8643 mL | 1.7285 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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