| Size | Price | Stock | Qty |
|---|---|---|---|
| 10g |
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| Other Sizes |
| ln Vitro |
- Uridine 5'-monophosphate disodium salt (UMP disodium salt) is a substrate of orotate phosphoribosyltransferase (OPRT). In human colon cancer HCT116 cells, exogenous addition of Uridine 5'-monophosphate disodium salt (100 μM) reversed the antiproliferative effect of 5-fluorouracil (5-FU). It increased cell viability by ~30% compared with the 5-FU-only group (5 μM 5-FU), as detected by MTT assay. This reversal was associated with supplementation of pyrimidine nucleotide pools, reducing 5-FU-mediated inhibition of DNA synthesis [1]
- In OPRT-overexpressing HCT116 cells, Uridine 5'-monophosphate disodium salt (50, 100, 200 μM) dose-dependently enhanced the resistance to 5-FU. At 200 μM, the IC50 of 5-FU increased from 3.2 μM (control) to 7.8 μM, indicating that UMP supplementation attenuates 5-FU cytotoxicity by competing with 5-FU metabolites for nucleotide incorporation [1] |
|---|---|
| Enzyme Assay |
- OPRT activity assay: Recombinant OPRT protein was incubated in reaction buffer containing Uridine 5'-monophosphate disodium salt (10-100 μM, as substrate analog control), orotate, and PRPP. The reaction was conducted at 37°C for 60 minutes and terminated by boiling. The production of uridine monophosphate (UMP) was quantified by high-performance liquid chromatography (HPLC) to evaluate OPRT catalytic activity [1]
|
| Cell Assay |
- 5-FU sensitivity modulation assay: HCT116 cells (wild-type or OPRT-overexpressing) were seeded in 96-well plates at 5×10³ cells/well and cultured overnight. Uridine 5'-monophosphate disodium salt (50-200 μM) was added 2 hours before 5-FU treatment (0.1-20 μM). Cells were incubated for 72 hours, and cell viability was measured by MTT assay. IC50 values of 5-FU were calculated to assess the effect of UMP on 5-FU sensitivity [1]
- OPRT expression correlation assay: HCT116 cells with OPRT knockdown or overexpression were treated with Uridine 5'-monophosphate disodium salt (100 μM) and 5-FU (5 μM) for 48 hours. Western blot was used to confirm OPRT expression levels, and flow cytometry was performed to detect apoptotic rates, analyzing the interaction between UMP, OPRT expression, and 5-FU-induced apoptosis [1] |
| References | |
| Additional Infomation |
Urate 5'-monophosphate (2-) is a pyrimidine nucleoside 5'-monophosphate (2-) formed by the removal of two protons from the phosphate group of UMP. It is a metabolite of both humans and Saccharomyces cerevisiae. It is a pyrimidine ribonucleotide 5'-monophosphate (2-), pyrimidine ribonucleotide, pyrimidine ribonucleotide monophosphate and ribonucleotide 5'-monophosphate. It is the conjugate base of uridine 5'-monophosphate.
- Urate 5'-monophosphate disodium salt is the disodium salt form of uridine 5'-monophosphate (UMP), which is a key intermediate in the biosynthesis of pyrimidine nucleotides [1] - In relevant literature, it is used as an experimental tool: as a substrate analog of OPRT, to study the relationship between OPRT expression and 5-FU sensitivity. It modulates the cytotoxicity of 5-FU by replenishing the intracellular UMP pool and competes with 5-FU metabolites (such as FUMP) in nucleotide metabolism [1] - The compound itself is not an anticancer drug, but is used to elucidate the OPRT-mediated 5-FU resistance mechanism. [1] |
| Molecular Formula |
C9H13N2NA2O9P
|
|---|---|
| Molecular Weight |
370.1608
|
| Exact Mass |
367.999
|
| CAS # |
3387-36-8
|
| Related CAS # |
Uridine 5'-monophosphate-13C9,15N2 disodium
|
| PubChem CID |
1778309
|
| Appearance |
White to off-white solid powder
|
| Density |
1.865g/cm3
|
| Melting Point |
208-210 °C
|
| Index of Refraction |
-14 ° (C=1, H2O)
|
| LogP |
-3.8
|
| Hydrogen Bond Donor Count |
3
|
| Hydrogen Bond Acceptor Count |
9
|
| Rotatable Bond Count |
3
|
| Heavy Atom Count |
21
|
| Complexity |
506
|
| Defined Atom Stereocenter Count |
4
|
| SMILES |
C1=CN(C(=O)NC1=O)[C@H]2[C@@H]([C@@H]([C@H](O2)COP(=O)([O-])[O-])O)O
|
| InChi Key |
DJJCXFVJDGTHFX-XVFCMESISA-L
|
| InChi Code |
InChI=1S/C9H13N2O9P/c12-5-1-2-11(9(15)10-5)8-7(14)6(13)4(20-8)3-19-21(16,17)18/h1-2,4,6-8,13-14H,3H2,(H,10,12,15)(H2,16,17,18)/p-2/t4-,6-,7-,8-/m1/s1
|
| Chemical Name |
[(2R,3S,4R,5R)-5-(2,4-dioxopyrimidin-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O : ≥ 50 mg/mL (~135.82 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (271.64 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.7015 mL | 13.5077 mL | 27.0153 mL | |
| 5 mM | 0.5403 mL | 2.7015 mL | 5.4031 mL | |
| 10 mM | 0.2702 mL | 1.3508 mL | 2.7015 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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