| Size | Price | Stock | Qty |
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| 1g |
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| Other Sizes |
| Targets |
Uniconazole targets cytochrome P450 707As (CYP707As), the major ABA catabolic enzymes. It acts as a potent and competitive inhibitor of CYP707A3 activity with a Ki of 8 nM. By inhibiting these enzymes, uniconazole prevents the breakdown of abscisic acid (ABA), leading to increased ABA levels. This, in turn, suppresses the biosynthesis of gibberellins and sterols, resulting in reduced shoot growth and other plant growth regulatory effects.
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| ln Vitro |
In vitro, Uniconazole has been shown to potently inhibit the activity of cytochrome P450 707As (CYP707As), the enzymes responsible for ABA catabolism. The compound has an IC50 of 68 nM for ABA 8'-hydroxylase and a Ki of 8 nM for CYP707A3. Uniconazole is a competitive inhibitor of these enzymes. The compound's inhibitory activity has been demonstrated in biochemical assays using recombinant CYP707A enzymes.
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| ln Vivo |
Uniconazole (0-50 μM) can effectively suppress ABA 8'-hydroxylase activity and promote drought tolerance in vivo [1]. Uniconazole can boost the activity of antioxidant enzymes, promote the accumulation of proline and soluble sugar, and prevent the buildup of malondialdehyde [2].
In vivo, Uniconazole is used as a plant growth regulator to reduce shoot growth and promote other desirable traits in plants. It is applied to various crops, including ornamental plants, fruits, and vegetables. Uniconazole has been shown to be effective in reducing plant height, increasing stem diameter, and promoting flowering. The compound's effects are mediated through the inhibition of gibberellin and sterol biosynthesis. |
| Enzyme Assay |
In vitro enzyme inhibition assays for Uniconazole typically involve measuring the activity of recombinant CYP707A enzymes in the presence of varying concentrations of the compound. The enzyme is incubated with its substrate, ABA, and Uniconazole. The production of the ABA catabolite is measured using HPLC or LC-MS. The IC50 value for inhibition of the enzyme is determined from dose-response curves. The Ki value is determined from kinetic analysis.
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| Cell Assay |
In vitro cell-based studies with Uniconazole are not typical, as the compound is primarily used as a plant growth regulator. However, its effects on plant cells can be studied. Plant cells or tissues are treated with Uniconazole at various concentrations, and the levels of ABA, gibberellins, and sterols are measured. The effect of Uniconazole on plant cell growth and differentiation can also be evaluated.
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| Animal Protocol |
Animal/Disease Models: Arabidopsis [1]
Doses: 0.5, 1, 5, 25, 50 μM Route of Administration: Experimental Results:Inhibition of PA (phyllic acid) production and enhanced drought tolerance. In vivo plant studies with Uniconazole are typically conducted in growth chambers or greenhouses. The compound is applied to plants as a soil drench, foliar spray, or seed treatment. The effect of Uniconazole on plant height, stem diameter, flowering, and other growth parameters is assessed. The levels of ABA, gibberellins, and sterols in plant tissues are measured. The efficacy of Uniconazole in reducing shoot growth and promoting other desirable traits is evaluated. |
| ADME/Pharmacokinetics |
The pharmacokinetic properties of Uniconazole in plants have been studied. The compound is absorbed by plant roots and leaves and is translocated to various tissues. Uniconazole is metabolized in plants to various metabolites. The compound's persistence in plants and soil varies depending on the application method and environmental conditions. Detailed PK parameters in plants have been reported in the literature.
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| Toxicity/Toxicokinetics |
The toxicity of Uniconazole has been evaluated in animals and environmental studies. The compound has a relatively low toxicity to mammals and birds. Uniconazole can be toxic to aquatic organisms. Appropriate safety precautions should be taken when handling the compound. Uniconazole is for agricultural use only and is not approved for human therapeutic use.
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| References |
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| Additional Infomation |
(1E)-1-(4-chlorophenyl)-4,4-dimethyl-2-(1H-1,2,4-triazol-1-yl)pent-1-en-3-ol is a triazole compound with a structure in which 1,2,4-triazole is substituted at the 1-position with a 1-(p-chlorophenyl)-3-hydroxy-4,4-dimethylpent-1-en-2-yl group. It belongs to the monochlorobenzene class, secondary alcohols, and triazole class.
Uniconazole is a plant growth regulator widely used in agriculture and horticulture. It is effective in reducing shoot growth, promoting flowering, and improving stress tolerance in various crops. Uniconazole is a member of the triazole class of plant growth regulators. The compound is not an FDA-approved drug and is not commercially available as a pharmaceutical product. |
| Molecular Formula |
C15H18CLN3O
|
|---|---|
| Molecular Weight |
291.78
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| Exact Mass |
291.113
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| CAS # |
83657-22-1
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| PubChem CID |
6436604
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| Appearance |
White to off-white solid powder
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| Density |
1.2±0.1 g/cm3
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| Boiling Point |
474.6±55.0 °C at 760 mmHg
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| Melting Point |
153ºC
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| Flash Point |
240.8±31.5 °C
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| Vapour Pressure |
0.0±1.2 mmHg at 25°C
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| Index of Refraction |
1.580
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| LogP |
3.84
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
20
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| Complexity |
346
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CC(C)(C)C(/C(=C\C1=CC=C(C=C1)Cl)/N2C=NC=N2)O
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| InChi Key |
YNWVFADWVLCOPU-MDWZMJQESA-N
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| InChi Code |
InChI=1S/C15H18ClN3O/c1-15(2,3)14(20)13(19-10-17-9-18-19)8-11-4-6-12(16)7-5-11/h4-10,14,20H,1-3H3/b13-8+
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| Chemical Name |
(E)-1-(4-chlorophenyl)-4,4-dimethyl-2-(1,2,4-triazol-1-yl)pent-1-en-3-ol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~342.72 mM)
H2O : < 0.1 mg/mL |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (8.57 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (8.57 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (8.57 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.4272 mL | 17.1362 mL | 34.2724 mL | |
| 5 mM | 0.6854 mL | 3.4272 mL | 6.8545 mL | |
| 10 mM | 0.3427 mL | 1.7136 mL | 3.4272 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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