| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| 50mg |
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| Other Sizes |
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| ln Vitro |
Within ten minutes of treating SH-SY5Y cells with GP130 receptor agonist-1 (compound 2), there was a two-fold increase in STAT3 phosphorylation at its regulatory Tyr705 site. At all times, pSTAT3 levels in primary hippocampus neuronal cells were lower than the amounts of GP130 receptor agonist-1 that could be detected [1]. In serum-free medium conditions, GP130 receptor agonist-1 treatment of SH-SY5Y cells and primary cortical neurons demonstrated phosphorylation of AKT at its regulatory Thr308 site and ERK1/2 at its Thr202/Tyr204 site. heightened dot phosphorylation [1].
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|---|---|
| ln Vivo |
Mice were given doses of 10 or 30 mg/kg or 10 mg/kg subcutaneously (SQ) of GP130 receptor agonist-1 (compound 2) at 1, 2, 4, and 6 p.i. and 8 hours after the dose of euthanasia. After two hours of treatment at 10 mg/kg SQ, the brain Cmax was 161 ng/g, and at 30 mg/kg oral dosage, it was 156 ng/g (0.57 μM). For oral dosage of 30 mg/kg and SQ injection of 10 mg/kg, the brain to plasma ratio of 2 is approximately 4:1 and 7.5:1, respectively [1].
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| References |
| Molecular Formula |
C15N2FSH11
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|---|---|
| Molecular Weight |
270.3246
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| Exact Mass |
270.062
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| CAS # |
339303-87-6
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| PubChem CID |
623055
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| Appearance |
White to off-white solid powder
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| Density |
1.3±0.1 g/cm3
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| Boiling Point |
424.2±47.0 °C at 760 mmHg
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| Flash Point |
210.3±29.3 °C
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| Vapour Pressure |
0.0±1.0 mmHg at 25°C
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| Index of Refraction |
1.660
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| LogP |
4.53
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
19
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| Complexity |
275
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
RSKMQDIAPGOWDL-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C15H11FN2S/c16-12-6-8-13(9-7-12)17-15-18-14(10-19-15)11-4-2-1-3-5-11/h1-10H,(H,17,18)
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| Chemical Name |
N-(4-fluorophenyl)-4-phenyl-1,3-thiazol-2-amine
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~369.93 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 2.5 mg/mL (9.25 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (9.25 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.6993 mL | 18.4966 mL | 36.9932 mL | |
| 5 mM | 0.7399 mL | 3.6993 mL | 7.3986 mL | |
| 10 mM | 0.3699 mL | 1.8497 mL | 3.6993 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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