| Size | Price | Stock | Qty |
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| 10mg |
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| 50mg |
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| 100mg |
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| 250mg | |||
| Other Sizes |
| Targets |
UAA crosslinker 1 hydrochloride does not have a biological target. It is a chemical reagent used for site-specific protein modification and crosslinking in biochemical research. The compound is an amber codon used for non-canonical amino acids (ncAAs) incorporation. The ncAAs can be incorporated into proteins in vivo by making use of the promiscuous activity of certain wildtype and engineered aminoacyl-tRNA synthetases. The compound contains an azide (N3) moiety that enables click chemistry reactions.
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| ln Vitro |
UAA crosslinker 1 hydrochloride is a chemical reagent and does not have intrinsic biological activity. The compound is used for site-specific protein modification and crosslinking in biochemical and structural biology research. It is an amber codon used for non-canonical amino acids (ncAAs) incorporation. The azide (N3) moiety enables click chemistry reactions (CuAAc) with compounds bearing an alkyne group. The compound's utility lies in its ability to facilitate the incorporation of non-canonical amino acids into proteins for studying protein structure and function.
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| ln Vivo |
UAA crosslinker 1 hydrochloride is a chemical reagent and is not used for in vivo pharmacological studies. The compound is used in biochemical and structural biology research for site-specific protein modification and crosslinking. It is an amber codon used for non-canonical amino acids (ncAAs) incorporation into proteins in vivo. The compound itself does not have in vivo activity; rather, it is a tool for studying protein structure and function through the incorporation of non-canonical amino acids.
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| Enzyme Assay |
UAA crosslinker 1 hydrochloride is a chemical reagent and does not have a biological assay protocol. The compound is used in chemical synthesis and protein engineering applications for site-specific protein modification and crosslinking. It is an amber codon used for non-canonical amino acids (ncAAs) incorporation. The azide (N3) moiety enables click chemistry reactions (CuAAc) with compounds bearing an alkyne group. Standard protocols for ncAA incorporation and click chemistry conjugation are used.
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| Cell Assay |
UAA crosslinker 1 hydrochloride is a chemical reagent and is not used directly in cell-based assays. The compound is used for site-specific protein modification and crosslinking in biochemical research. It is an amber codon used for non-canonical amino acids (ncAAs) incorporation into proteins in cells. Cells are engineered to express orthogonal tRNA/tRNA synthetase pairs that incorporate ncAAs in response to an amber codon. The incorporated ncAAs can then be used for site-specific protein labeling or crosslinking.
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| Animal Protocol |
UAA crosslinker 1 hydrochloride is a chemical reagent and is not used directly in animal studies. The compound is used for non-canonical amino acids (ncAAs) incorporation into proteins in vivo. Animals are engineered to express orthogonal tRNA/tRNA synthetase pairs that incorporate ncAAs in response to an amber codon. The incorporated ncAAs can then be used for site-specific protein labeling or crosslinking in vivo. The compound itself is not administered as a therapeutic agent.
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| ADME/Pharmacokinetics |
UAA crosslinker 1 hydrochloride has a molecular weight of 295.72 and a molecular formula of C9H18ClN5O4. The compound is an amber codon used for non-canonical amino acids (ncAAs) incorporation. It contains an azide (N3) moiety and can undergo click chemistry reactions (CuAAc) with compounds bearing an alkyne group. The compound is soluble in DMSO and water. It should be stored at -20degC. The compound has a purity of ≥97-98%.
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| Toxicity/Toxicokinetics |
UAA crosslinker 1 hydrochloride is a chemical reagent and comprehensive toxicology data are not applicable. The compound is intended for research use only and is not approved for human therapeutic applications. Standard laboratory safety practices should be followed when handling this compound, including the use of appropriate personal protective equipment and adherence to institutional biosafety and chemical hygiene guidelines.
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| Additional Infomation |
UAA crosslinker 1 hydrochloride is a synthetic chemical probe designed for site-specific protein modification and crosslinking in biochemical and structural biology research. It is an amber codon used for non-canonical amino acids (ncAAs) incorporation. The compound contains an azide (N3) moiety and can undergo click chemistry reactions (CuAAc) with compounds bearing an alkyne group. UAA crosslinker 1 hydrochloride is for research purposes only.
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| Molecular Formula |
C9H18CLN5O4
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|---|---|
| Molecular Weight |
295.723320484161
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| Exact Mass |
295.104
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| CAS # |
1994331-17-7
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| Related CAS # |
UAA crosslinker 1;1167421-25-1
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| PubChem CID |
102571564
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| Appearance |
Off-white to light yellow solid powder
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
10
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| Heavy Atom Count |
19
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| Complexity |
316
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| Defined Atom Stereocenter Count |
1
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| SMILES |
C(CCNC(=O)OCCN=[N+]=[N-])C[C@@H](C(=O)O)N.Cl
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| InChi Key |
USQKPJVGUFZIJQ-FJXQXJEOSA-N
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| InChi Code |
InChI=1S/C9H17N5O4.ClH/c10-7(8(15)16)3-1-2-4-12-9(17)18-6-5-13-14-11;/h7H,1-6,10H2,(H,12,17)(H,15,16);1H/t7-;/m0./s1
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| Chemical Name |
(2S)-2-amino-6-(2-azidoethoxycarbonylamino)hexanoic acid;hydrochloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~125 mg/mL (~422.70 mM)
H2O : ~100 mg/mL (~338.16 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (7.03 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.08 mg/mL (7.03 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (7.03 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.3816 mL | 16.9079 mL | 33.8158 mL | |
| 5 mM | 0.6763 mL | 3.3816 mL | 6.7632 mL | |
| 10 mM | 0.3382 mL | 1.6908 mL | 3.3816 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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