| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| ln Vivo |
In the forced swimming and tail suspension tests, young rats given tyrosylleucine (YL) orally, intracerebroventricularly, or intraperitoneally show strong antidepressant-like effects [1].
|
|---|---|
| Animal Protocol |
Animal/Disease Models: Male ddY mouse (24-27 g) [1]
Doses: 0.1-30 mg/kg, intraperitoneal (ip) injection; 0.1-1.0 nmol/mouse, icv; 30-100 mg/kg, oral Route of Administration: Results of po (po (oral gavage)) intracerebroventricular, or intraperitoneal Route of Administration: diminished immobility time after intraperitoneal(1-30 mg/kg) and intracerebroventricular (0.3-1 nmol/mouse) administration in the FST. Demonstrated potent antidepressant-like activity in forced swimming and tail suspension tests in young rats. |
| References |
[1]. Takafumi Mizushige, et al. Dipeptide tyrosyl-leucine exhibits antidepressant-like activity in mice. Sci Rep. 2020 Feb 10;10(1):2257.
[2]. Norimasa Kanegawa, et al. Dipeptide Tyr-Leu (YL) exhibits anxiolytic-like activity after oral administration via activating serotonin 5-HT1A, dopamine D1 and GABAA receptors in mice. FEBS Lett. 2010 Feb 5;584(3):599-604. |
| Additional Infomation |
Tyr-Leu is a dipeptide formed from L-tyrosine and L-leucine residues. It has a role as a metabolite.
|
| Molecular Formula |
C15H22N2O4
|
|---|---|
| Molecular Weight |
294.35
|
| Exact Mass |
294.158
|
| CAS # |
17355-10-1
|
| Related CAS # |
Tyrosylleucine TFA;66852-01-5
|
| PubChem CID |
87071
|
| Appearance |
Typically exists as solid at room temperature
|
| Density |
1.218g/cm3
|
| Boiling Point |
575.9ºC at 760 mmHg
|
| Flash Point |
302.1ºC
|
| Vapour Pressure |
4.2E-14mmHg at 25°C
|
| Index of Refraction |
1.565
|
| LogP |
1.968
|
| Hydrogen Bond Donor Count |
4
|
| Hydrogen Bond Acceptor Count |
5
|
| Rotatable Bond Count |
7
|
| Heavy Atom Count |
21
|
| Complexity |
352
|
| Defined Atom Stereocenter Count |
2
|
| SMILES |
CC(C)C[C@H](NC([C@@H](N)CC1=CC=C(O)C=C1)=O)C(O)=O
|
| InChi Key |
AUEJLPRZGVVDNU-STQMWFEESA-N
|
| InChi Code |
InChI=1S/C15H22N2O4/c1-9(2)7-13(15(20)21)17-14(19)12(16)8-10-3-5-11(18)6-4-10/h3-6,9,12-13,18H,7-8,16H2,1-2H3,(H,17,19)(H,20,21)/t12-,13-/m0/s1
|
| Chemical Name |
(2S)-2-[[(2S)-2-amino-3-(4-hydroxyphenyl)propanoyl]amino]-4-methylpentanoic acid
|
| Synonyms |
L-Tyrosyl-L-leucine; Tyr-leu; Tyrosylleucine
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O : ~250 mg/mL (~849.33 mM)
DMSO : ~250 mg/mL (~849.33 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (7.07 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (7.07 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (7.07 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.3973 mL | 16.9866 mL | 33.9732 mL | |
| 5 mM | 0.6795 mL | 3.3973 mL | 6.7946 mL | |
| 10 mM | 0.3397 mL | 1.6987 mL | 3.3973 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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